产品应用
应用说明
ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 1 μg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user. GTX18664 will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 Protein. No reaction can be seen with synthetic NS-3.
产品说明
GTX18665 reacts with recombinant NS-3 (amino acids 1252-1477), synthetic NS-3 (amino acids 1378-1458), and secombinant chimeric HCV polyprotein (60 kDa). No cross reaction can be seen with recombinant CPC* + EPM** (core) (amino acids 1-142), synthetic CPC* (amino acids 1-61), and synthetic NS-4a protein (amino acids 1689-1735).
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Hepatitis C virus
免疫原
Purified recombinant chimeric HCV polyprotein (555 amino acids).
纯化方式
Protein G purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Hepatitis C Virus Serine protease NS3 , Hepatitis C Virus NS3 , HCV Serine protease NS3 , HCV NS3
细胞定位
Cell Membrane, Cytoplasmic and Plasma membrane
功能与背景
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
研究领域
文献引用
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产品评论
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