产品应用
应用说明
ELISA: Use at an assay dependent dilution. IHC-P: The dilution fold should be from 1:100 to 1:1000. WB: Use at a concentration of 0.5 μg/ml to detect 0.5 μg/lane of synthetic NS4 peptide and 0.1 μg/lane recombinant chimeric HCV polyprotein. Optimal dilutions/concentrations should be determined by the end user.
产品说明
This antibody reacts with synthetic NS4a Protein, and recombinant Chimeric HCV Polyprotein. No cross-reaction with HCV capsid region and other non-structure regions.
产品属性
形式
Liquid
存储溶液
0.01M PBS pH7.0
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Hepatitis C virus
免疫原
Synthetic peptide (Human) in which overlapping sequences of at least 90 residues in length were selected from the immunodominant NS4 regions of Chinese HCV strains.
纯化方式
Protein G purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Hepatitis C Virus Non-structural protein 4A , Hepatitis C Virus NS4A , HCV Non-structural protein 4A , HCV NS4A
细胞定位
RNA virus
功能与背景
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
研究领域
文献引用
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产品评论
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