产品说明
摘要
HER2 antibody recognizes HER2 protein, also known as proto-oncogene Neu or ERBB2 (predicted molecular weight of 138 kDa). HER2 is a member of the human epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. It shares 50% sequence identity with EGFR protein, but involves different signaling pathways. HER2 can heterodimerize with EGFR protein or homodimerize when it is present in high concentrations.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:20000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HCT116 , A549 , H1299 , HCT116 , MCF-7
预测种属反应
Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.07 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of human Her2 / ErbB2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10618490
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1
细胞定位
Membrane; Single-pass type I membrane protein
功能与背景
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX100509 IHC-P Image
Her2 / ErbB2 antibody detects Her2 / ErbB2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Green: Her2 / ErbB2 stained by Her2 / ErbB2 antibody (GTX100509) diluted at 1:50.
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GTX100509 WB Image
Wild-type (WT) and Her2 / ErbB2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody (GTX100509) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX100509 IHC-P Image
Her2 / ErbB2 antibody [C2C3], C-term detects Her2 / ErbB2 protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Strong positive (++), low positive (+) and negative tissue slides cores assessed using Quantitative Digital Pathology. Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:500, and competitor's antibody (CST#4290) diluted at 1:100.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX100509 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100509 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody (GTX100509) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100509 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100509 IHC-P Image
Her2 / ErbB2 antibody detects Her2 / ErbB2 protein at cell membrane by immunohistochemical analysis.
Sample: Human Breast Cancer.
Green: Her2 / ErbB2 stained by Dylight488-conjugated Her2 / ErbB2 antibody (GTX100509) diluted at 1:500.
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GTX100509 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:10000.
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文献引用
产品评论
Her2 / ErbB2 antibody
目录号 GTX100509
Rating
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( Average 5 based on 2 users reviews)
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实验应用
IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 18-Oct-2021
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
human breast cancer
Primary Antibody :
1:50, 4°C, 16Hr
Date :
Anonymous submitted on 16-Jul-2021
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
Breast cancer tissue
Primary Antibody :
1:500, 4°C, 16Hr