产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Mouse, Bovine, Cat, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1248 of human Her2 / ErbB2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1
细胞定位
Nucleus
功能与背景
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940](/templates/default/js/lightbox/images/loading.gif "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940")
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GTX636940 IHC-P Image
Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636940 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636940 ICC/IF Image
Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein at cell membrane by immunofluorescent analysis.Sample: Mock and treated A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636940 IHC-P Image
Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940](/upload/website/prouct_img/small/GTX636940/GTX636940_44774_20221227_IHC-P_multiple_R_22122821_449.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940")
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Western Blot (WB). GTX636940](/upload/website/prouct_img/small/GTX636940/GTX636940_44774_20220819_WB_treatment_EGF_22082402_614.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Western Blot (WB). GTX636940")
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636940](/upload/website/prouct_img/small/GTX636940/GTX636940_T-44662_20230106_ICC_IF_treatment_peptide blocking_23013122_771.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636940")
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940](/upload/website/prouct_img/small/GTX636940/GTX636940_T-44662_20220701_IHC-P_22070501_754.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940")
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940](/upload/website/prouct_img/normal/GTX636940/GTX636940_44774_20221227_IHC-P_multiple_R_22122821_449.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940")
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Western Blot (WB). GTX636940](/upload/website/prouct_img/normal/GTX636940/GTX636940_44774_20220819_WB_treatment_EGF_22082402_614.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Western Blot (WB). GTX636940")
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636940](/upload/website/prouct_img/normal/GTX636940/GTX636940_T-44662_20230106_ICC_IF_treatment_peptide blocking_23013122_771.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636940")
![Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940](/upload/website/prouct_img/normal/GTX636940/GTX636940_T-44662_20220701_IHC-P_22070501_754.webp "Anti-Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] used in IHC (Paraffin sections) (IHC-P). GTX636940")
