产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:500 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 150mM NaCl, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthesized peptide derived from Human ERBB2(Accession P04626), corresponding to amino acid residues P1228-V1255.
纯化方式
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1
细胞定位
Nucleus
功能与背景
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX03358 WB Image
WB analysis of serum starvation treated MCF7 cell lysate using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody. The lane on the left was treated with blocking peptide.
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GTX03358 IHC-P Image
IHC-P analysis of human TB tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 IHC-P Image
IHC-P analysis of human brain tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 IHC-P Image
IHC-P analysis of mouse brain tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 IHC-P Image
IHC-P analysis of rat appendiceal tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 IHC-P Image
IHC-P analysis of mouse testicular tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 IHC-P Image
IHC-P analysis of human skin tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 IHC-P Image
IHC-P analysis of rat ovarian tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX03358 IHC-P Image
IHC-P analysis of rat kidney tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 IHC-P Image
IHC-P analysis of mouse gastric tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX03358 WB Image
WB analysis of various sample lysates using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat spleen tissue lysate treated with blocking peptide.
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GTX03358 ICC/IF Image
ICC/IF analysis of PFA-fixed MDA-MB-231 cells using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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