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Hexokinase II抗体

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525
Anti-Hexokinase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111525
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

目录号 GTX111525

文献引用 9
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Hexokinase II 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

102 kDa. ( Note )

预测种属反应

Zebrafish, Chicken, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.62 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Hexokinase II. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10724113

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

hexokinase 2 , HKII , HXK2

细胞定位

Mitochondrion outer membrane

功能与背景

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

GTX111525 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

GTX111525 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hexokinase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111525

GTX111525 ICC/IF Image

Hexokinase II antibody detects Hexokinase II protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

GTX111525 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35328751

文献引用

Application Reference

Izabela Bary?a et al. Int J Mol Sci 2022; 23 (6) The WWOX/HIF1A Axis Downregulation Alters Glucose Metabolism and Predispose to Metabolic Disorders.
实验应用 : WB
种属反应 : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
实验应用 : WB
种属反应 : Human
Harada Y et al. Cell Rep 2020; 33 (2) : 108261 Glycometabolic Regulation of the Biogenesis of Small Extracellular Vesicles.
实验应用 : WB
种属反应 : Mouse
Lee YR et al. Kaohsiung J Med Sci 2020; 36 (11) : 911-919 Regulation of autophagy, glucose uptake, and glycolysis under dengue virus infection.
实验应用 : WB
种属反应 : Human
Takino JI et al. Sci Rep 2019; 9 (1) : 16717 The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells.
实验应用 : WB
种属反应 : Human
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产品评论

Hexokinase II antibody 目录号 GTX111525

Rating
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实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525
Date : Anonymous submitted on 23-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : Placenta tissue lysate
Amount used : 60 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
我要评论产品
Application Reference
Izabela Bary?a et al. Int J Mol Sci 2022; 23 (6) The WWOX/HIF1A Axis Downregulation Alters Glucose Metabolism and Predispose to Metabolic Disorders.
实验应用 : WB
种属反应 : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
实验应用 : WB
种属反应 : Human
Harada Y et al. Cell Rep 2020; 33 (2):108261 Glycometabolic Regulation of the Biogenesis of Small Extracellular Vesicles.
实验应用 : WB
种属反应 : Mouse
Lee YR et al. Kaohsiung J Med Sci 2020; 36 (11):911-919 Regulation of autophagy, glucose uptake, and glycolysis under dengue virus infection.
实验应用 : WB
种属反应 : Human
Takino JI et al. Sci Rep 2019; 9 (1):16717 The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells.
实验应用 : WB
种属反应 : Human
Lee CH et al. Mol Imaging Biol 2018; (Epub) Adenine Nucleotide Translocase 2 as an Enzyme Related to [18F] FDG Accumulation in Various Cancers.
实验应用 : WB
种属反应 : Human
Yang T et al. Oncogene 2018; (Epub) pim2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and * resistance in breast cancer.
实验应用 : WB
种属反应 : Human
Huang YP et al. Clin Exp Pharmacol Physiol 2017; (Epub) Proteomic analysis of oral cancer reveals new potential therapeutic targets involved in the Warburg effect.
实验应用 : WB
种属反应 : Human
Jan CI et al. Int J Biol Sci? 2016; 12 (7):786-798 Fenofibrate Suppresses Oral Tumorigenesis via Reprogramming Metabolic Processes: Potential Drug Repurposing for Oral Cancer.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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