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Histone H2A.XS139ph (phospho Ser139)抗体

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

目录号 GTX127342

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

种属反应

Human, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:2000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:2000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

15 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Zebrafish

免疫原

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of zebrafish Histone H2A.X. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2833105

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

H2A histone family, member X , zgc:56329

细胞定位

Nucleus

功能与背景

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

数据库

研究领域

產品图片

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342

GTX127342 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342

GTX127342 IHC Image

H2afx antibody detects H2afx protein at nucleus on UV-irradiated whole-mount zebrafish embryos (under panel) by immunohistochemical analysis.
Sample: H2afx antibody (GTX127342) dilution: 1:2000.
Image provided with permission courtesy of Dr. James Amatruda.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342

GTX127342 WB Image

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on UV-irradiated whole mount zebrafish (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 days-post-fertilization zebrafish embryo.
Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342

GTX127342 ICC/IF Image

The data was published in the journal Dis Model Mech in 2018.PMID: 29419415

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342

GTX127342 ICC/IF Image

The data was published in the journal Dis Model Mech in 2018.PMID: 29419415

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

The data was published in the journal Sci Rep in 2019. PMID: 31882844

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

The data was published in the journal Nat Commun in 2019. PMID: 30837464

文献引用

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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