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Histone H3抗体

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148
Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148

目录号 GTX122148

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

种属反应

Human, Mouse, Rat, Drosophila, Pig, Monkey, Fungi, Golden Syrian Hamster, Rice
产品包装
100 μl (¥3590),
25 μl (¥1390)
产品说明

摘要

Histone H3 antibody detects histone H3, a core component of the histone octamer in the context of the nucleosome. Like other histones, H3 is posttranslationally modified to impact epigenetic gene regulation. It is also highly conserved in eukaryotes, and therefore is used as an internal control for western blots or as a nuclear marker when conducting immunostaining.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

15 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

产品属性

形式

Liquid

存储溶液

1XPBS (pH7), 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10633308

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: Histone H3 antibody (GTX122148) diluted at 1:500.

Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3(GTX122148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148

GTX122148 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Histone H3 antibody detects Histone H3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at a dilution of 1:1000.

Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148

GTX122148 IP Image

Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1:5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148

GTX122148 ICC/IF Image

Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Histone H3 protein stained by Histone H3 antibody (GTX122148) diluted at 1:500.
Blue: Hoechst 33342 staining.

文献引用

产品评论

Histone H3 antibody 目录号 GTX122148

Rating ( Average 4.4 based on 9 users reviews)
实验应用
Western Blot(WB) ( Average 4.2 based on 6 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 22-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW1353 cells
Amount used : 25μg
Blocking : Block Ace, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2014. PMID: 25393278
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017. PMID: 28855246
Date : Anonymous submitted on 27-Dec-2020
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse lung tissue
Blocking : 4% BSA in PBS, 25°C, 1Hr
Fixation : Formaldehide
Permeabilization /Antigen retrieval : Citrate buffer, pH6.0, 90°C, 0.6Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Histone H3 accumulation in nucleus is shown in brown. Rabbit IgG is used as negative control.
Date : Anonymous submitted on 05-Oct-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 05-Oct-2019
Score :
Application Tested : WB
Sample Species : Golden Syrian Hamster
Sample : BHK21 cells
Amount used : 5 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 23-May-2014
Score :
Application Tested : WB
Sample Species : Rice
Sample : rice leaf tissue
Amount used : 50μg
Blocking : 5% milk in tris buffer, 4°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
Date : Anonymous submitted on 10-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : primary adipocyte cells
Amount used : 10μg
Blocking : 5% nonfat milk in TBST(1xTBS, 0.1%Tween 20), 25°C, 3Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 22-Dec-2012
Score :
Application Tested : WB
Sample Species : Mk
Sample : Vero E6 cells
Amount used : 100μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 3590
¥ 1390
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