Hsp90 alpha抗体

Name: Hsp90 alpha抗体
Brand: GeneTex
SKU: GTX109753
Price: 4000.00 RMB
Rating: 5 (4 reviews)

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX109753

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753

Anti-Hsp90 alpha antibody used in Immunoprecipitation (IP). GTX109753

Anti-Hsp90 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109753

目录号 GTX109753

文献引用 36

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP
种属反应	Human, Mouse, Rat, Drosophila, Monkey, Nematode
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX109753 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

85 kDa. ( Note )

阳性对照

mouse brain , rat brain , 293T , A431 , HeLa , HepG2

产品说明

Knockdown/Knockout validation was supported by customer review data.

预测种属反应

Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Hsp90 alpha. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11178231

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

heat shock protein 90 alpha family class A member 1 , EL52 , HEL-S-65p , HSP86 , HSP89A , HSP90A , HSP90N , HSPC1 , HSPCA , HSPCAL1 , HSPCAL4 , HSPN , Hsp103 , Hsp89 , Hsp90 , LAP-2 , LAP2

细胞定位

Cytoplasm , Melanosome

功能与背景

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

数据库

Gene ID: 3320 HSP90AA1
UniProt: P07900 HSP90AA1

研究领域

產品图片

GTX109753 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109753 IP Image

Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 μg of Hsp90 alpha antibody (GTX109753).
Western blot analysis was performed using Hsp90 alpha antibody (GTX109753).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109753 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Hsp90 alpha antibody (GTX109753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109753 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Hsp83 is the homolog of mammalian Hsp90. (Gene ID: 38389/ UniProt: P02828)

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109753 ICC/IF Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109753 WB Image

The data was published in the journal PLoS One in 2016. PMID: 26863132

GTX109753 WB Image

The data was published in the journal Cells in 2019. PMID: 31905895

GTX109753 WB Image

The data was published in the journal Elife in 2019.PMID: 31284899

GTX109753 WB Image

The data was published in the journal Elife in 2019.PMID: 31284899

GTX109753 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30846715

GTX109753 WB Image

The data was published in the journal Elife in 2019.PMID: 31284899

GTX109753 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30846715

文献引用

Application Reference

Y Wu et al. Metabolites 2024; 14 (1) : 51 Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response

实验应用 : WB

种属反应 : Human

SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23) : 00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B

实验应用 : WB, ICC/IF

种属反应 : Human

Lista MJ et al. J Virol 2023; 97 (1) : e0087222 A Nuclear Export Signal in KHNYN Required for Its Antiviral Activity Evolved as ZAP Emerged in Tetrapods.

Lista MJ et al. J Virol 2022; 96 (23) : e0125022 The P681H Mutation in the Spike Glycoprotein of the Alpha Variant of SARS-CoV-2 Escapes IFITM Restriction and Is Necessary for Type I Interferon Resistance.

Pariollaud M et al. Sci Adv 2022; 8 (39) : eabo1123 Circadian disruption enhances HSF1 signaling and tumorigenesis in <i>Kras</i>-driven lung cancer.

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产品评论

Hsp90 alpha antibody 目录号 GTX109753

Rating

( Average 5 based on 4 users reviews)

实验应用

Western Blot(WB)		( Average 5 based on 3 users reviews)
Immunoprecipitation(IP)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 02-Mar-2018

Score :

Application Tested : WB

Sample Species : Hu

Sample : PC-3 cells

Blocking : 5% ECL Blocking Agent, 25°C, 1Hr

Primary Antibody : 1:5000, 4°C, 16Hr

条带说明 : Lane 1: WCL 10 μg, Lane 2: heat shock (43oC 1.5h) WCL 4 μg, Lane 3: heat shock (43oC 3.0h) WCL 8 μg

Date : Anonymous submitted on 26-Jul-2017

Score :

Application Tested : WB

Sample Species : Ms

Sample : MC3T3-E1 cells

Amount used : 50 μg

Blocking : 5% milk in TBST, 25°C, 0.5Hr

Primary Antibody : 1:2000, 4°C, 18Hr

Date : Anonymous submitted on 26-Apr-2017

Score :

Application Tested : WB

Sample Species : Hu

Sample : HT29 cells

Blocking : 5% skim milk in PBST, 25°C, 1Hr

Primary Antibody : 1:5000, 4°C, 16Hr

条带说明 : Lane1: HT29 shLacZ (virus infection to silence LacZ gene), Lane2: HT29 shHSP90AA1 ( virus infection to silence HSP90AA1 gene)

Date : Anonymous submitted on 18-Jan-2013

Score :

Application Tested : IP

Sample Species : Rat

Sample : RK3E cells

Amount used : 50μg

Blocking : 5% non-fat milk, 25°C, 1Hr

Primary Antibody : 1:5000, 4°C, 16Hr

Figure Note : 2ug for IP

我要评论产品

Application Reference

Y Wu et al. Metabolites 2024; 14 (1):51 Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response

实验应用 : WB

种属反应 : Human

SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23):00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B

实验应用 : WB, ICC/IF

种属反应 : Human

Lista MJ et al. J Virol 2023; 97 (1):e0087222 A Nuclear Export Signal in KHNYN Required for Its Antiviral Activity Evolved as ZAP Emerged in Tetrapods.

Lista MJ et al. J Virol 2022; 96 (23):e0125022 The P681H Mutation in the Spike Glycoprotein of the Alpha Variant of SARS-CoV-2 Escapes IFITM Restriction and Is Necessary for Type I Interferon Resistance.

Pariollaud M et al. Sci Adv 2022; 8 (39):eabo1123 Circadian disruption enhances HSF1 signaling and tumorigenesis in <i>Kras</i>-driven lung cancer.

Xiaowei Song et al. Front Microbiol 2021; 12:799890 Hsp90 Inhibitors Inhibit the Entry of Herpes Simplex Virus 1 Into Neuron Cells by Regulating Cofilin-Mediated F-Actin Reorganization.

实验应用 : WB

种属反应 : Mouse

Hang Yuan et al. Int J Mol Sci 2021; 22 (16) PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.

实验应用 : WB

种属反应 : Human

Winstone H et al. J Virol 2021; 95 (9) The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2.

实验应用 : WB

种属反应 : Human, Monkey

Lin TY et al. Phytomedicine 2021; 80:153384 Functional proteomic analysis reveals that fungal immunomodulatory protein reduced expressions of heat shock proteins correlates to apoptosis in lung cancer cells.

实验应用 : WB

种属反应 : Mouse

Ono K et al. J Extracell Vesicles 2020; 9 (1):1769373 Triple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer.

Gunin AG et al. Adv Gerontol 2020; 33 (1):40-45 [Heat shock protein 90 (HSP90) in age-dependent changes in the number of fibroblasts in human skin.]

Kok BP et al. Nat Chem Biol 2020; 16 (9):997-1005 Discovery of small-molecule enzyme activators by activity-based protein profiling.

实验应用 : WB

种属反应 : Human

Eguchi T et al. Cells 2020; 9 (3) Cell Stress Induced Stressome Release Including Damaged Membrane Vesicles and Extracellular HSP90 by Prostate Cancer Cells.

实验应用 : WB

种属反应 : Human

Cai H et al. Int J Mol Med 2020; (Epub)

实验应用 : WB

种属反应 : Rat

Chua KV et al. Cells 2019; 9 (1) Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90帢 Secretion, and Tumor Growth.

实验应用 : WB

种属反应 : Human

Ficarelli M et al. Elife 2019; 8 KHNYN is essential for the zinc finger antiviral protein (ZAP) to restrict HIV-1 containing clustered CpG dinucleotides.

实验应用 : WB

种属反应 : Human

Qin F et al. J Enzyme Inhib Med Chem 2019; 34 (1):909-926 Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.

实验应用 : WB

种属反应 : Human

Suzuki S et al. Sci Rep 2019; 9 (1):3825 Dental pulp cell-derived powerful inducer of TNF-α comprises PKR containing stress granule rich microvesicles.

实验应用 : WB

种属反应 : Human

Shahabi S et al. Cancer Res 2019; (Epub) LINC00261 is an epigenetically-regulated tumor suppressor that is essential for activation of the DNA damage response.

实验应用 : WB

Chen CC et al. Oncoimmunology 2018; 7 (5):e1424612 Myeloid-derived macrophages and secreted HSP90α induce pancreatic ductal adenocarcinoma development.

Iida R et al. Oxid Med Cell Longev 2018; 2018:6956414 Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels.

Pei-Ying Li et al. RSC Adv. 2018; 8:31515-31528 Alpinumisoflavone attenuates lipopolysaccharide-induced acute lung injury by regulating the effects of anti-oxidation and anti-inflammation both in vitro and.

实验应用 : WB

种属反应 : Mouse

Yuan SH et al. Hum Mol Genet 2018; (Epub) Tauopathy-Associated PERK Alleles are Functional Hypomorphs that Increase Neuronal Vulnerability to ER Stress.

实验应用 : WB

种属反应 : Mouse

Ono K et al. J Cell Biochem 2018; (Epub) HSP-enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells.

实验应用 : WB

种属反应 : Human

Fan CS et al. Oncotarget 2018; 9 (4):4998-5015 Osteopontin-integrin engagement induces HIF-1α-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer.

实验应用 : WB

种属反应 : Human

Luu BE et al. Cell Stress Chaperones 2017; The regulation of heat shock proteins in response to dehydration in Xenopus laevis.

实验应用 : WB

Chen X et al. Redox Biol 2017; 12:987-1003 The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.

实验应用 : WB

种属反应 : Rat

Chiang WC et al. Proc Natl Acad Sci U S A 2017; 114 (2):400-405 Achromatopsia mutations target sequential steps of ATF6 activation.

实验应用 : WB

种属反应 : Human

Tung H et al. PLoS One 2016; 11 (2):e0148578 Baculovirus IE2 Stimulates the Expression of Heat Shock Proteins in Insect and Mammalian Cells to Facilitate Its Proper Functioning.

Wu DW et al. Cell Death Dis 2016; 7 (6):e2290 TC-N19, a novel dual inhibitor of EGFR and cMET, efficiently overcomes EGFR-TKI resistance in non-small-cell lung cancer cells.

实验应用 : WB

种属反应 : Human

Succoio M et al. J Proteomics 2015; 128 (Epub):18-29 Proteomic analysis reveals novel common genes modulated in both replicative and stress-induced senescence.

实验应用 : WB

种属反应 : Human

Chen HY et al. Vet Parasitol 2015; 212 (43163):281-91 Expression patterns and structural modelling of Hsp70 and Hsp90 in a fish-borne zoonotic nematode Anisakis pegreffii.

实验应用 : WB

Hiramatsu N et al. Mol Biol Cell 2014; 25 (9):1411-20 Translational and posttranslational regulation of XIAP by eIF2α and ATF4 promotes ER stress-induced cell death during the unfolded protein response.

实验应用 : WB

种属反应 : Human

Chen HY et al. Vet Parasitol 2014; Effect of different temperatures on the expression of the newly characterized heat shock protein 90 (Hsp90) in L3 of Anisakis spp. isolated from Scomber australasicus.

实验应用 : WB

种属反应 : Nematode

Lee WY et al. Toxicol Appl Pharmacol 2014; 274 (1):55-62 The induction of heme oxygenase-1 suppresses heat shock protein 90 and the proliferation of human breast cancer cells through its byproduct carbon monoxide.

实验应用 : WB

种属反应 : Human

Liu SH et al. Biochem Pharmacol 2014; 87 (2):229-42 Andrographolide downregulates the v-Src and Bcr-Abl oncoproteins and induces Hsp90 cleavage in the ROS-dependent suppression of cancer malignancy.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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Hsp90 alpha抗体

目录号 GTX109753

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品说明

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX109753 WB Image

GTX109753 IHC-P Image

GTX109753 IP Image

GTX109753 WB Image

GTX109753 IHC-P Image

GTX109753 WB Image

GTX109753 IHC-P Image

GTX109753 ICC/IF Image

GTX109753 IHC-P Image

GTX109753 IHC-P Image

GTX109753 WB Image

GTX109753 WB Image

GTX109753 WB Image

GTX109753 WB Image

GTX109753 WB Image

GTX109753 WB Image

GTX109753 WB Image

文献引用

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产品评论

Hsp90 alpha antibody 目录号 GTX109753

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