产品说明
摘要
Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins.
产品应用
应用说明
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED₅₀:: <1ng/ml).
产品说明
Binds to human CD40.
产品属性
形式
Lyophilized powder
存储溶液
Reconstitute with 100μl ddH₂O to 0.1mg/ml. Lyophilized from 0.2μm-filtered PBS.
保存剂
No preservative
存放说明
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
Region/Sequence
N-terminal DDDDK-Tag; Human CD40L (a.a.116-261) is fused at the N-terminus to mouse ACRP30headless (a.a.18-111).
表达系统
CHO cells
纯度
??95% by SDS-PAGE.
内毒素
< 0.01 EU/μg
偶联
Unconjugated
注意事项
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
相关讯息
别名
CD40 ligand , CD154 , CD40L , HIGM1 , IGM , IMD3 , T-BAM , TNFSF5 , TRAP , gp39 , hCD40L
数据库
研究领域
產品图片
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GTX65635-pro Image
GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100μl RPMI supplemented with 10% FCS) for 24 hours at 37ºC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1μg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
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产品评论
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