产品应用
应用说明
Specific activity is > 300 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37ºC.
Observed MW
28-40 kDa.
Default 3
7.4
产品属性
形式
Liquid
存储溶液
PBS, 10% Glycerol
保存剂
No preservative
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
C-terminal His-Tag; Length: 283 a.a. Sequence: APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS
ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG
FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV
CTAAGLSHHH HHH
表达系统
Baculovirus
纯度
> 90% by SDS-PAGE.
内毒素
< 1.0 EU/μg (determined by LAL method)
偶联
Unconjugated
注意事项
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
相关讯息
别名
carbonic anhydrase 12 , CA-XII , CAXII , HsT18816 , T18816
功能与背景
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]
数据库
研究领域
產品图片
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GTX66937-pro Image
3 μg of GTX66937-pro Human Carbonic Anhydrase XII protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain
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文献引用
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产品评论
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