产品应用
应用说明
Troponin I Type 3, Cardiac (TNNI3) is a protein that in humans is encoded by the TNNI3 gene. It is a tissue-specific subtype of troponin I, which in turn is a part of the troponin complex. The TNNI3 gene encoding cardiac troponin I (cTnI) is located at 19q13.4 in the human chromosomal genome. Human cTnI is a 24kDa protein consisting of 210 amino acids with isoelectric point (pI) of 9.87. cTnI is exclusively expressed in adult cardiac muscle. Besides, Troponin T Type 2, Cardiac (TNNT2) has been identified as an interactor of TNNI3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNI3 and recombinant human TNNT2. Briefly, TNNI3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNNT2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNNI3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNNI3 and TNNT2 was in a dose dependent manner.
Observed MW
28 kDa.
Default 3
8
产品属性
形式
Lyophilized powder
存储溶液
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
保存剂
ProClin 300
存放说明
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Met1~Gly203 (NP_000354.4)
表达系统
E. coli
纯度
> 97%
内毒素
< 1 EU/μg
偶联
Unconjugated
注意事项
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
相关讯息
别名
troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI
功能与背景
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX00237-pro Functional Assay Image
Functional ELISA analysis of GTX00237-pro Human Cardiac Troponin I protein which can bind immobilized TNNT2 protein.
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GTX00237-pro Image
WB analysis of GTX00237-pro Human Cardiac Troponin I protein.
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GTX00237-pro Image
SDS-PAGE analysis of GTX00237-pro Human Cardiac Troponin I protein.
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产品评论
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