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Human IL18 蛋白, His tag

Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro
Human IL18 protein, His tag. GTX00227-pro

目录号 GTX00227-pro

实验应用

ELISA, Functional Assay

Speciess

Human
产品包装
10 μg (¥4000)

产品应用

应用说明

Recommended antibody pairs for sandwich ELISA:
Capture : GTX637584, Detection : GTX637411 or Capture : GTX637411, Detection : GTX637584.
The detection antibody requires HRP conjugation. Please contact us for the conjugation service.

Observed MW

18 kDa.

Default 3

8

产品属性

形式

Lyophilized powder

存储溶液

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

保存剂

ProClin 300

存放说明

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Tyr37~Asp193 (NP_001230140.1)

表达系统

E. coli

纯度

> 90%

内毒素

< 1 EU/μg

偶联

Unconjugated

注意事项

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

相关讯息

别名

interleukin 18 , IGIF , IL-18 , IL-1g , IL1F4

功能与背景

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

数据库

研究领域

產品图片

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Functional Assay Image

Functional ELISA analysis of GTX00227-pro Human IL18 protein which can bind immobilized CASP1 protein.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL)
Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1859] (GTX637584) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

SDS-PAGE analysis of GTX00227-pro Human IL18 protein.

Human IL18 protein, His tag. GTX00227-pro

GTX00227-pro Image

WB analysis of GTX00227-pro Human IL18 protein.

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SDS
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
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