产品应用
应用说明
MCP-1 (monocyte chemoattractant protein 1), also known as CCL2 (C-C motif chemokine 2), is a small cytokine that belongs to the CC chemokine family. MCP-1 has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of MCP-1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 10⁶ cells/ml in RPMI-1640 with 0.5% FBS) and MCP-1 (25 and 50 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). MCP-1 is able to induce migration of THP-1 cells, and the optimum chemotactic concentration of MCP-1 is about 25 ng/ml.
Observed MW
15/17 kDa.
产品属性
形式
Lyophilized powder
存储溶液
Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
保存剂
ProClin 300
存放说明
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Gln24~Thr99 (NP_002973.1)
表达系统
E. coli
纯度
> 90%
内毒素
< 1 EU/μg
偶联
Unconjugated
注意事项
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
相关讯息
别名
C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF
功能与背景
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
数据库
研究领域
產品图片
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GTX00415-pro Functional Assay Image
The chemotactic effect of GTX00415-pro Human MCP1 / CCL2 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 25 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(C) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 50 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).
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GTX00415-pro Image
WB analysis of GTX00415-pro Human MCP1 / CCL2 protein (active).
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GTX00415-pro Image
SDS-PAGE analysis of GTX00415-pro Human MCP1 / CCL2 protein (active).
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文献引用
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产品评论
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