Human MCP1 / CCL2 蛋白, His tag (active)

The chemotactic effect of GTX00415-pro Human MCP1 / CCL2 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 25 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(C) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 50 ng/ml MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

SDS-PAGE analysis of GTX00415-pro Human MCP1 / CCL2 protein (active).

WB analysis of GTX00415-pro Human MCP1 / CCL2 protein (active).