Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (1 μg/mL)
Please notice that GTX637228 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (1 μg/mL)
Please notice that GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637228. Please contact us for custom HRP-conjugated antibody.

Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Human Papillomavirus type 16 E7 antibody (GTX637228 [HL1647])
Detection: Human Papillomavirus type 16 E7 antibody (GTX637546 [HL1821])

Samples (10 ng) :
1. HPV16 E7 protein (GTX133411-pro)
2. HPV18 E7 protein (GTX133412-pro)
3. Lysis buffer