Non-transfected (–) and transfected (+) U87-MG whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000.

Wild-type (WT) and HTT knockout (KO) 293T cell extracts (10 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Huntingtin antibody (GTX132433) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.