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ICAM1 / CD54抗体 [N1C2]

Orthogonal
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

目录号 GTX100450

文献引用 14
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ICAM1 / CD54 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

58 kDa. ( Note )

Observed MW

58 or 85~110 kDa. The observed M.W. is based on the following publication. PMID: 24474243

预测种属反应

Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human ICAM1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950536

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

intercellular adhesion molecule 1 , BB2 , CD54 , P3.58

细胞定位

Membrane; Single-pass type I membrane protein

功能与背景

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100450

GTX100450 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ICAM1 / CD54(GTX100450) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25675397

Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450

GTX100450 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25675397

文献引用

Application Reference

YJ Shi et al. Eur J Pharmacol 2023; Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction
Vos S et al. Invest Ophthalmol Vis Sci 2023; 64 (7) : 17 CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression.
Wu CY et al. J Cell Mol Med 2023; 27 (11) : 1509-1522 CX3CL1 induces cell migration and invasion through ICAM-1 expression in oral squamous cell carcinoma cells.
Chang TM et al. Int J Biol Sci 2023; 19 (5) : 1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
实验应用 : WB
种属反应 : Human
Chang MC et al. Int Endod J 2021; (Epub) Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.
实验应用 : WB
种属反应 : Human
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ICAM1 / CD54 antibody [N1C2] 目录号 GTX100450

Rating
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实验应用
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 : Lane 1: Untreated MG63 whole cell lysate, Lane 2: treated (TSP-2; 3ug/mL) MG63 whole cell lysate, Lane 3: treated (TSP-2; 10ug/mL) MG63 whole cell lysate
Anti-ICAM1 / CD54 antibody [N1C2] used in Western Blot (WB). GTX100450
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
Application Reference
YJ Shi et al. Eur J Pharmacol 2023; Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction
Vos S et al. Invest Ophthalmol Vis Sci 2023; 64 (7):17 CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression.
Wu CY et al. J Cell Mol Med 2023; 27 (11):1509-1522 CX3CL1 induces cell migration and invasion through ICAM-1 expression in oral squamous cell carcinoma cells.
Chang TM et al. Int J Biol Sci 2023; 19 (5):1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
实验应用 : WB
种属反应 : Human
Chang MC et al. Int Endod J 2021; (Epub) Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.
实验应用 : WB
种属反应 : Human
Chang WC et al. Plants (Basel) 2021; 10 (7) Vescalagin from Pink Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Protects Pancreatic β-Cells against Methylglyoxal-Induced Inflammation in Rats.
实验应用 : WB
种属反应 : Rat
Corgnac S et al. Cell Rep Med 2020; 1 (7):100127 CD103+CD8+ TRM Cells Accumulate in Tumors of Anti-PD-1-Responder Lung Cancer Patients and Are Tumor-Reactive Lymphocytes Enriched with Tc17.
实验应用 : IHC-P
种属反应 : Human
Huang FI et al. Front Oncol 2019; 9:249 MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells.
Sol? M et al. Biochim Biophys Acta Mol Basis Dis 2019; (Epub) Blood-brain barrier dysfunction underlying Alzheimer's disease is induced by an SSAO/VAP-1-dependent cerebrovascular activation with enhanced Aβ deposition.
实验应用 : WB
种属反应 : Human
Chang MC et al. J Formos Med Assoc 2018; (Epub) IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38.
实验应用 : WB
种属反应 : Human
Sun P et al. Biochim Biophys Acta 2017; 1864 (2):542-553 Simvastatin blocks soluble SSAO/VAP-1 release in experimental models of cerebral ischemia: Possible benefits for stroke-induced inflammation control.
实验应用 : WB
种属反应 : Human
Lee TH et al. PLoS One 2015; Collagen inhibitory peptide R1R2 mediates vascular remodeling by decreasing inflammation and smooth muscle cell activation.
实验应用 : WB
种属反应 : Human
Claude S et al. Mol Nutr Food Res 2014; 58 (5):1016-27 Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.
实验应用 : WB
种属反应 : Human
Koya T et al. Am J Physiol Heart Circ Physiol 2012; 303 (1):H96-105 Salusin-β accelerates inflammatory responses in vascular endothelial cells via NF-κB signaling in LDL receptor-deficient mice in vivo and HUVECs in vitro.
实验应用 : WB
种属反应 : Human
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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