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IDH2抗体 [GT673]

KO/KD Overexpression
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487
Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487
Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

目录号 GTX628487

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT673

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 IDH2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

51 kDa. ( Note )

阳性对照

rat brain , Jurkat , Raji , K562 , THP-1 , NCI-H929 , mouse brain , DDDDK-tagged IDH2-transfected 293T , HepG2

预测种属反应

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888040

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial , D2HGA2 , ICD-M , IDH , IDHM , IDP , IDPM , mNADP-IDH

细胞定位

Mitochondrion

功能与背景

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody [GT673] detects IDH2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
10% SDS-PAGE
IDH2 antibody [GT673] (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

GTX628487 ICC/IF Image

IDH2 antibody detects IDH2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: IDH2 protein stained by IDH2 antibody (GTX628487) diluted at 1:500.
Red: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody detects IDH2 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg THP-1 whole cell lysate/extract
E. 30 μg NCI-H929 whole cell lysate/extract
10% SDS-PAGE
IDH2 antibody (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487

GTX628487 IHC-P Image

IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
IDH2 antibody [GT673] (GTX628487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDH2 antibody [GT673] used in IHC (Paraffin sections) (IHC-P). GTX628487

GTX628487 IHC-P Image

IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IDH2 antibody [GT673] (GTX628487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

IDH2 antibody [GT673] detects IDH2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
12% SDS-PAGE
IDH2 antibody [GT673] (GTX628487) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Western Blot (WB). GTX628487

GTX628487 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDH2 antibody [GT673] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628487

GTX628487 ICC/IF Image

IDH2 antibody [GT673] detects IDH2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: IDH2 protein stained by IDH2 antibody [GT673] (GTX628487) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

文献引用

Application Reference

Liu TF et al. J Biol Chem 2015; 290 (1) : 396-408 Sequential actions of SIRT1-RELB-SIRT3 coordinate nuclear-mitochondrial communication during immunometabolic adaptation to acute inflammation and sepsis.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
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¥ 1700
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