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IGF1R beta抗体 [HL1957]

Recombinant antibody KO/KD Orthogonal
Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794
Anti-IGF1R beta antibody [HL1957] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637794
Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794
Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794
Anti-IGF1R beta antibody [HL1957] used in IHC (Paraffin sections) (IHC-P). GTX637794
Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794

目录号 GTX637794

1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1957

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 IGF1R 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

155 kDa. ( Note )

产品说明

This antibody was raised against human IGF1R Extracellular domain.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human IGF1R. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

细胞定位

Cell membrane

功能与背景

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

数据库

研究领域

產品图片

Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794

GTX637794 WB Image

KO/KD

Wild-type (WT) and IGF1R knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IGF1R beta antibody [HL1957] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637794

GTX637794 ICC/IF Image

IGF1R beta antibody [HL1957] detects IGF1R beta protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1R beta stained by IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794

GTX637794 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794

GTX637794 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF1R beta antibody [HL1957] used in IHC (Paraffin sections) (IHC-P). GTX637794

GTX637794 IHC-P Image

IGF1R beta antibody [HL1957] detects IGF1R protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IGF1R beta stained by IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794

GTX637794 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品评论

IGF1R beta antibody [HL1957] 目录号 GTX637794

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : BxPC-3 cells with/without shIGF1R treatment
Amount used : 15 μg
Blocking : 1% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 16Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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