产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human IGF2 protein, and it does not cross react with human IGF1, INS-IGF2, and INS protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-term region of human IGF2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974
细胞定位
Secreted
功能与背景
This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
数据库
研究领域
產品图片
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/templates/default/js/lightbox/images/loading.gif "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
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GTX638104 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638104 WB Image
Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638104 ELISA Image
Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL2132] (GTX638104) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX638104 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230331_WB_Fraction_23041023_266.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230414_WB_Ag_23041723_301.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230512_ELISA_Indirect_23051702_722.webp "Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230526_WB_multiple_B_23053001_192.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230331_WB_Fraction_23041023_266.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230414_WB_Ag_23041723_301.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230512_ELISA_Indirect_23051702_722.webp "Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230526_WB_multiple_B_23053001_192.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
