产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Neuro2A whole , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12
预测种属反应
Bovine, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.86 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human IL1 Receptor antagonist. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950581
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
interleukin 1 receptor antagonist , DIRA , ICIL-1RA , IL-1RN , IL-1ra , IL-1ra3 , IL1F3 , IL1RA , IRAP , MVCD4
细胞定位
Isoform 1: Secreted , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 4: Cytoplasm
功能与背景
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX106490 IHC-P Image
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist(GTX106490) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106490 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL1 Receptor antagonist antibody (GTX106490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX106490 WB Image
IL1 Receptor antagonist antibody detects IL1 Receptor antagonist protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with IL1 Receptor antagonist antibody (GTX106490) diluted by 1:1000
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GTX106490 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist (GTX106490) antibody at 1:200 dilution.
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GTX106490 WB Image
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106490 WB Image
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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