GeneTex中国官方网站
  • China

IL1 beta抗体 [GT289]

Orthogonal
Anti-IL1 beta antibody [GT289] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634188
Anti-IL1 beta antibody [GT289] used in Western Blot (WB). GTX634188

目录号 GTX634188

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT289

同种型

 IgG2a

实验应用

 WB, ICC/IF

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 IL1 beta 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

31 kDa. ( Note )

阳性对照

THP-1 (500 ng/mL LPS treatment for 6 hr and then 300 ng/mL Brefeldin treatment for the last 3 hr)

预测种属反应

Rabbit, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL1 beta. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888424

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

细胞定位

Secreted

功能与背景

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-IL1 beta antibody [GT289] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634188

GTX634188 ICC/IF Image

Orthogonal

IL1 beta antibody [GT289] detects IL1 beta protein by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody [GT289] (GTX634188) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody [GT289] used in Western Blot (WB). GTX634188

GTX634188 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [GT289] (GTX634188) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Julien Stackowicz et al. J Exp Med 2021; 218 (10) Neutrophil-specific gain-of-function mutations in Nlrp3 promote development of cryopyrin-associated periodic syndrome.
我要回报文献

产品评论

目前没有使用 IL1 beta antibody [GT289] (GTX634188) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com