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IL1 beta抗体 [HL1421]

Recombinant antibody Orthogonal
Anti-IL1 beta antibody [HL1421] used in Western Blot (WB). GTX636887
Anti-IL1 beta antibody [HL1421] used in IHC (Paraffin sections) (IHC-P). GTX636887
Anti-IL1 beta antibody [HL1421] used in IHC (Paraffin sections) (IHC-P). GTX636887
Anti-IL1 beta antibody [HL1421] used in IHC (Paraffin sections) (IHC-P). GTX636887
Anti-IL1 beta antibody [HL1421] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636887
Anti-IL1 beta antibody [HL1421] used in ELISA (ELISA). GTX636887
Anti-IL1 beta antibody [HL1421] used in ELISA (ELISA). GTX636887

目录号 GTX636887

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1421

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, ELISA

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX636889/GTX636887, Detection: GTX636887/GTX636889
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

31 kDa. ( Note )

产品说明

The use of GTX636887 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX74034.

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

细胞定位

Cytoplasm, cytosol,Lysosome,Secreted

功能与背景

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-IL1 beta antibody [HL1421] used in Western Blot (WB). GTX636887

GTX636887 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [HL1421] (GTX636887) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 beta antibody [HL1421] used in IHC (Paraffin sections) (IHC-P). GTX636887

GTX636887 IHC-P Image

IL1 beta antibody [HL1421] detects secreted IL1 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 beta antibody [HL1421] used in IHC (Paraffin sections) (IHC-P). GTX636887

GTX636887 IHC-P Image

IL1 beta antibody [HL1421] detects secreted IL1 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 beta antibody [HL1421] used in IHC (Paraffin sections) (IHC-P). GTX636887

GTX636887 IHC-P Image

IL1 beta antibody [HL1421] detects IL1 beta protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 beta antibody [HL1421] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636887

GTX636887 ICC/IF Image

IL1 beta antibody [HL1421] detects IL1 beta protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody [HL1421] used in ELISA (ELISA). GTX636887

GTX636887 ELISA Image

Sandwich ELISA detection of recombinant IL1 beta using antibodies as below.

Capture: IL1 beta antibody [HL1421] (GTX636887) (5 μg/mL)
Detection: HRP-conjugated IL1 beta antibody [HL1423] (GTX636889) (1 μg/mL)
Please notice that GTX636889 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636887. Please contact us for custom HRP-conjugated antibody.

Anti-IL1 beta antibody [HL1421] used in ELISA (ELISA). GTX636887

GTX636887 ELISA Image

Sandwich ELISA detection of recombinant IL1 beta using antibodies as below.

Capture: IL1 beta antibody [HL1423] (GTX636889) (5 μg/mL)
Detection: HRP-conjugated IL1 beta antibody [HL1421] (GTX636887) (1 μg/mL)
Please notice that GTX636887 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636889. Please contact us for custom HRP-conjugated antibody.

文献引用

Application Reference

Chiou WC et al. Biomed Pharmacother 2023; 161 : 114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
实验应用 : IHC-P
种属反应 : Mouse
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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