产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Note :
Sandwich ELISA
Capture: GTX636889/GTX636887, Detection: GTX636887/GTX636889.
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WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
The use of GTX636889 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX74034.
预测种属反应
Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
细胞定位
Cytoplasm, cytosol,Lysosome,Secreted
功能与背景
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX636889 ELISA Image
Sandwich ELISA detection of recombinant IL1 beta using antibodies as below. Capture: IL1 beta antibody [HL1423] (GTX636889) (5 μg/mL) Detection: HRP-conjugated IL1 beta antibody [HL1421] (GTX636887) (1 μg/mL)Please notice that GTX636887 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636889. Please contact us for custom HRP-conjugated antibody.
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GTX636889 ELISA Image
Sandwich ELISA detection of recombinant IL1 beta using antibodies as below. Capture: IL1 beta antibody [HL1421] (GTX636887) (5 μg/mL) Detection: HRP-conjugated IL1 beta antibody [HL1423] (GTX636889) (1 μg/mL)Please notice that GTX636889 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636887. Please contact us for custom HRP-conjugated antibody.
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GTX636889 ICC/IF Image
IL1 beta antibody [HL1423] detects IL1 beta protein at cytoplasm by immunofluorescent analysis.Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636889 WB Image
Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636889 IHC-P Image
IL1 beta antibody [HL1423] detects secreted IL1 beta protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.IL1 beta stained by IL1 beta antibody [HL1423] (GTX636889) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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