应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000 |
| 1:1:100-1:200 |
| 1:1:100-1:200 |
| Assay dependent |
| 1:400-1:800 |
| 1:500-1:2000 |
| 1:100 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions.The antiserum does not recognize human IL-1alpha.
形式
Liquid
存储溶液
20mM Potassium Phosphate, 150mM NaCl
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
This antibody was prepared by repeated immunizations with recombinant human IL-1s produced in E.coli. The MW of the recombinant 153 aa IL-1s was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1s.
纯化方式
Purified by DEAE chromatography.
From serum
偶联
Unconjugated
RRID
AB_368268
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
别名
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
细胞定位
Cytoplasm, cytosol,Lysosome,Secreted
功能与背景
Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
数据库
研究领域
