应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
3x Flag human IL-10-transfected 293T
预测种属反应
Rabbit, Sheep, Bovine, Cat, Guinea pig, Chimpanzee, Rhesus Monkey(>80% identity)
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.19 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human IL10. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888308
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
别名
interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF
细胞定位
Secreted
功能与背景
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]
数据库
研究领域
![Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359](/upload/website/prouct_img/small/GTX632359/GTX632359_42079_20170929_IHC-P_M_w_23061202_342.webp "Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359")
![Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359](/upload/website/prouct_img/small/GTX632359/GTX632359_42079_20150508_WB_B_w_23061202_444.webp "Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359")
![Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359](/upload/website/prouct_img/small/GTX632359/GTX632359_42079_20170929_IHC-P_R_w_23061202_837.webp "Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359")
![Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359](/upload/website/prouct_img/small/GTX632359/GTX632359_20240411_citeab_WB_8_24041501_577.webp "Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359")
![Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359](/upload/website/prouct_img/normal/GTX632359/GTX632359_42079_20170929_IHC-P_M_w_23061202_342.webp "Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359")
![Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359](/upload/website/prouct_img/normal/GTX632359/GTX632359_42079_20150508_WB_B_w_23061202_444.webp "Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359")
![Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359](/upload/website/prouct_img/normal/GTX632359/GTX632359_42079_20170929_IHC-P_R_w_23061202_837.webp "Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359")
![Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359](/upload/website/prouct_img/normal/GTX632359/GTX632359_20240411_citeab_WB_8_24041501_577.webp "Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359")
