产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
IL17A-transfected 293T , mouse spleen , rat spleen
预测种属反应
Bovine, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.15 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human IL17A. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2887108
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
interleukin 17A , CTLA-8 , CTLA8 , IL-17 , IL-17A , IL17
细胞定位
Secreted
功能与背景
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX133781 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membranes were blotted with IL17A antibody (GTX133781) diluted at 1:1000 and competitor's antibody (sc-7927) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
GTX133781 WB Image
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with IL17A antibody (GTX133781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
GTX133781 IHC-P Image
IL17A antibody detects IL17A protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.IL17A stained by IL17A antibody (GTX133781) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX133781 IHC-P Image
IL17A antibody detects IL17A protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon.IL17A stained by IL17A antibody (GTX133781) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX133781 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL17A antibody (GTX133781) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
文献引用
产品评论
目前没有使用 IL17A antibody (GTX133781) 的产品评论被收录。快发表第一篇产品评论吧!