GeneTex中国官方网站
  • China

IL6抗体 [HL2389]

Recombinant antibody Comparison Orthogonal
Anti-IL6 antibody [HL2389] used in Western Blot (WB). GTX638598
Anti-IL6 antibody [HL2389] used in IHC (Paraffin sections) (IHC-P). GTX638598
Anti-IL6 antibody [HL2389] used in ELISA (ELISA). GTX638598
Anti-IL6 antibody [HL2389] used in IHC (Paraffin sections) (IHC-P). GTX638598
Anti-IL6 antibody [HL2389] used in Western Blot (WB). GTX638598
Anti-IL6 antibody [HL2389] used in Western Blot (WB). GTX638598
Anti-IL6 antibody [HL2389] used in ELISA (ELISA). GTX638598
Anti-IL6 antibody [HL2389] used in ELISA (ELISA). GTX638598

目录号 GTX638598

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2389

同种型

 IgG

实验应用

 WB, IHC-P, ELISA, Sandwich ELISA

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 IL6 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636571 / GTX636573, Detection : GTX638598
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

24 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human IL6.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

细胞定位

Secreted

功能与背景

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

数据库

研究领域

產品图片

Anti-IL6 antibody [HL2389] used in Western Blot (WB). GTX638598

GTX638598 WB Image

Comparison

Recombinant IL6 were separated by 15% SDS-PAGE, and the membranes were blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IL6 antibody [HL2389] used in IHC (Paraffin sections) (IHC-P). GTX638598

GTX638598 IHC-P Image

IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL6 antibody [HL2389] used in ELISA (ELISA). GTX638598

GTX638598 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-IL6 antibody [HL2389] used in IHC (Paraffin sections) (IHC-P). GTX638598

GTX638598 IHC-P Image

IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL6 antibody [HL2389] used in Western Blot (WB). GTX638598

GTX638598 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IL6 antibody [HL2389] used in Western Blot (WB). GTX638598

GTX638598 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IL6 antibody [HL2389] used in ELISA (ELISA). GTX638598

GTX638598 ELISA Image

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

Anti-IL6 antibody [HL2389] used in ELISA (ELISA). GTX638598

GTX638598 ELISA Image

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

文献引用

目前没有使用 IL6 antibody [HL2389] (GTX638598) 的文献被收录。快来回报我们第一篇参考文献吧！
我要回报文献

产品评论

目前没有使用 IL6 antibody [HL2389] (GTX638598) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com