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ILK抗体 [N1C1]

KO/KD
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691
Anti-ILK antibody [N1C1] used in Immunohistochemistry (IHC). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in Immunoprecipitation (IP). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

目录号 GTX101691

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, IHC-Wm, IP, IHC

种属反应

 Human, Mouse, Rat, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ILK 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

51 kDa. ( Note )

阳性对照

A431 , Neuro2A , C8D30 , NIH-3T3 , C2Cl2 , PC-12 , Neuro 2A , C8D30 , Raw264.7 , HeLa

预测种属反应

Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950586

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

integrin linked kinase , HEL-S-28 , ILK-1 , ILK-2 , P59 , p59ILK

细胞定位

Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side

功能与背景

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

KO/KD

Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ILK antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX101691

GTX101691 IHC-Wm Image

Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish muscle
10% SDS PAGE
GTX101691 diluted at 1:1000

Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691

GTX101691 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1] (GTX101691) at 1:300 dilution.

Anti-ILK antibody [N1C1] used in Immunohistochemistry (IHC). GTX101691

GTX101691 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ILK antibody [N1C1] (GTX101691) at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691

GTX101691 IHC-P Image

ILK antibody [N1C1] detects ILK protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ILK antibody [N1C1] (GTX101691) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

ILK antibody [N1C1] detects ILK protein by western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw 264.7 whole cell extract
E. 30 μg C2Cl2 whole cell extract
10 % SDS-PAGE
ILK antibody [N1C1] (GTX101691) dilution: 1:1000

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX101691 diluted at 1:500

Anti-ILK antibody [N1C1] used in Immunoprecipitation (IP). GTX101691

GTX101691 IP Image

ILK antibody [N1C1] immunoprecipitates ILK protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of ILK protein by 4 μg ILK antibody [N1C1] (GTX101691)
10 % SDS-PAGE
The immunoprecipitated ILK protein was detected by ILK antibody [N1C1] (GTX101691) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

文献引用

Application Reference

Murase H et al. Oncol Rep 2023; 50 (3)
H Murase et al. Oncology Reports 2023; 50 : 164
实验应用 : WB
Chen YL et al. Sci Rep 2021; 11 (1) : 644 Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells.
实验应用 : WB
种属反应 : Human
Wantoch von Rekowski K et al. Biomolecules 2019; 9 (12)
实验应用 : WB
种属反应 : Human
Yen YC et al. Oncotarget 2015; 6 (39) : 41837-55 *-like growth factor-independent *-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin β1.
实验应用 : WB
种属反应 : Human
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