产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human ISG15
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ISG15 ubiquitin like modifier , G1P2 , IFI15 , IMD38 , IP17 , UCRP , hUCRP
细胞定位
Cytoplasm,Secreted
功能与背景
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
数据库
研究领域
產品图片
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/templates/default/js/lightbox/images/loading.gif "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
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GTX637918 WB Image
Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637918 IHC-P Image
ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat brain.ISG15 stained by ISG15 antibody [HL2017] (GTX637918) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637918 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637918 WB Image
Untreated (–) and treated (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637918 ICC/IF Image
ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: ISG15 stained by ISG15 antibody [HL2017] (GTX637918) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX637918 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/small/GTX637918/GTX637918_T-44872_20221223_WB_KO_watermark_22122722_661.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
![Anti-ISG15 antibody [HL2017] used in IHC (Paraffin sections) (IHC-P). GTX637918](/upload/website/prouct_img/small/GTX637918/GTX637918_44963_20230804_IHC-P_Cat_23090619_460.webp "Anti-ISG15 antibody [HL2017] used in IHC (Paraffin sections) (IHC-P). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/small/GTX637918/GTX637918_T-44872_20221223_WB_M_R_22122722_181.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/small/GTX637918/GTX637918_44963_20230224_WB_treatment_IFN-gamma_23030219_978.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637918](/upload/website/prouct_img/small/GTX637918/GTX637918_T-44872_20230210_ICC_IF_23021401_491.webp "Anti-ISG15 antibody [HL2017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/small/GTX637918/GTX637918_44963_20230616_WB_C_23062019_137.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/normal/GTX637918/GTX637918_T-44872_20221223_WB_KO_watermark_22122722_661.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
![Anti-ISG15 antibody [HL2017] used in IHC (Paraffin sections) (IHC-P). GTX637918](/upload/website/prouct_img/normal/GTX637918/GTX637918_44963_20230804_IHC-P_Cat_23090619_460.webp "Anti-ISG15 antibody [HL2017] used in IHC (Paraffin sections) (IHC-P). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/normal/GTX637918/GTX637918_T-44872_20221223_WB_M_R_22122722_181.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/normal/GTX637918/GTX637918_44963_20230224_WB_treatment_IFN-gamma_23030219_978.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637918](/upload/website/prouct_img/normal/GTX637918/GTX637918_T-44872_20230210_ICC_IF_23021401_491.webp "Anti-ISG15 antibody [HL2017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637918")
![Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918](/upload/website/prouct_img/normal/GTX637918/GTX637918_44963_20230616_WB_C_23062019_137.webp "Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918")
