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ITCH抗体 [GT1248]

Recombinant antibody KO/KD
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

目录号 GTX02845

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 GT1248

同种型

 IgG

实验应用

 WB

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥5600)
查看所有 ITCH 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500 - 1:2000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

103 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 0.05% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.6 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

A synthesized peptide derived from human ITCH

纯化方式

Purified by antigen-affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

itchy E3 ubiquitin protein ligase , ADMFD , AIF4 , AIP4 , NAPP1

细胞定位

Cell membrane , Cytoplasm , Nucleus , Endosome membrane

功能与背景

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

数据库

研究领域

產品图片

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

WB analysis of various samples using GTX02845 ITCH antibody [GT1248].
Dilution : 1:1000
Loading : 25μg

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

WB analysis of various samples using GTX02845 ITCH antibody [GT1248].
Dilution : 1:1000
Loading : 25μg

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

KO/KD

WB analysis of wide type (WT) and ITCH knockout (KO) 293T cells using GTX02845 ITCH antibody [GT1248].
Dilution : 1:1000
Loading : 25μg

文献引用

Application Reference

Hsiao-Chin Chou et al. J Biomed Sci 2022; 29 (1) : 40 DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.
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产品包装 报价 (¥)
¥ 5600
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