产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Iba1
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1
细胞定位
Cytoplasm, cytoskeleton
功能与背景
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
数据库
研究领域
產品图片
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GTX637629 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL1880] (GTX637629) diluted at 1:1000 and competitor's antibody (CST17198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX637629 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL1880] (GTX637629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637629 ICC/IF Image
Iba1 antibody [HL1880] detects Iba1 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637629 IHC-Fr Image
Iba1 antibody [HL1880] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Frozen-sectioned mouse brain.Green: Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637629 IHC-P Image
Iba1 antibody [HL1880] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637629 IHC-P Image
Iba1 antibody [HL1880] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637629 IHC-Fr Image
Iba1 antibody [HL1880] detects Iba1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse spinal cord.Green: Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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