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Iba1抗体 [HL22-MS]

Recombinant antibody Orthogonal
Anti-Iba1 antibody [HL22-MS] used in IHC (Frozen sections) (IHC-Fr). GTX635399
Anti-Iba1 antibody [HL22-MS] used in IHC (Paraffin sections) (IHC-P). GTX635399
Anti-Iba1 antibody [HL22-MS] used in Western Blot (WB). GTX635399

目录号 GTX635399

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 HL22-MS

同种型

 IgG1

实验应用

 WB, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Iba1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500
IHC-P 1:500
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

17 kDa. ( Note )

预测种属反应

Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888527

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

细胞定位

Cytoplasm, cytoskeleton

功能与背景

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

数据库

研究领域

產品图片

Anti-Iba1 antibody [HL22-MS] used in IHC (Frozen sections) (IHC-Fr). GTX635399

GTX635399 IHC-Fr Image

Iba1 antibody [HL22-MS] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22-MS] (GTX635399) diluted at 1:25.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: ice-cold MeOH for 5 min

Anti-Iba1 antibody [HL22-MS] used in IHC (Paraffin sections) (IHC-P). GTX635399

GTX635399 IHC-P Image

Iba1 antibody [HL22-MS] detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22-MS] (GTX635399) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22-MS] used in Western Blot (WB). GTX635399

GTX635399 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22-MS] (GTX635399) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

文献引用

Application Reference

Chu PC et al. Neurotherapeutics 2023; 20 (2) : 502-517 Pulsed Focused Ultrasound Reduces Hippocampal Volume Loss and Improves Behavioral Performance in the Kainic Acid Rat Model of Epilepsy.
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PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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