产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500 |
Assay dependent |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888528
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1
细胞定位
Cytoplasm, cytoskeleton
功能与背景
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
数据库
研究领域
產品图片
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GTX635400 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX635400 ICC/IF Image
Iba1 antibody [HL22-RT] detects Iba1 protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX635400 IHC-P Image
Iba1 antibody [HL22-RT] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635400 IHC-P Image
Iba1 antibody [HL22-RT] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635400 IHC-Fr Image
Iba1 antibody [HL22-RT] detects Iba1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse brain.Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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