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Influenza A virus M2 (matrix protein)抗体

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

目录号 GTX125951

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, EM

种属反应

Influenza A virus
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
EM Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

11 kDa. ( Note )

阳性对照

WSN-infected DF-1 cell

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Influenza A virus

免疫原

Recombinant protein corresponding to Influenza A virus M2 (matrix protein) (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11170983

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Influenza A Virus Membrane 2 protein , Influenza A Virus M2

研究领域

產品图片

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951

GTX125951 ICC/IF Image

Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: M2 protein stained by Influenza A Virus M2 antibody (GTX125951) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Sample (50 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
15% SDS PAGE
GTX125951 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody (GTX125951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal Front Microbiol in 2017. PMID: 29312159

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal J Virol in 2017.PMID: 27795429

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal PLoS Pathog in 2018.PMID: 29723291

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal J Virol in 2017.PMID: 27795429

文献引用

产品评论

Influenza A virus M2 (matrix protein) antibody 目录号 GTX125951

Rating ( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
条带说明 : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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