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Influenza A virus Nucleoprotein抗体 [HL1089]

Recombinant antibody Comparison
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636247

目录号 GTX636247

文献引用 3
2

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1089

同种型

 IgG

实验应用

 WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)

种属反应

 Influenza A virus
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Influenza A virus Nucleoprotein 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Lateral Flow
Capture : GTX637790, Detection : GTX636247 or Capture : GTX636247 / GTX636318, Detection : GTX636199 or Capture : GTX636247 / GTX636199, Detection : GTX636318. Please notice that GTX636247 needs to be conjugated to Gold to function as the detection antibody when paired with GTX637790. Please contact us for custom Gold-conjugated antibody.

Sandwich ELISA
Capture : GTX636318 / GTX636247, Detection : GTX636247 / GTX636318. Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318 / GTX637790. Please contact us for custom HRP-conjugated antibody.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody is specific for Influenza A virus Nucleoprotein protein (H1N1, H3N2, H5N8, and H10N3), and it does not cross-react with Influenza B virus Nucleoprotein protein.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Influenza A virus

免疫原

Recombinant protein encompassing a sequence within the center region of Influenza A virus Nucleoprotein (A/Kansas/14/2017(H3N2)). The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909956

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247

GTX636247 Lateral Flow Image

Detection of recombinant influenza A virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
Detection: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating a plate with recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag (GTX135903-pro) (50 ng), and probing with the specified influenza A virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza A virus or influenza B virus and probing with the specified influenza A virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247

GTX636247 ICC/IF Image

Influenza A virus Nucleoprotein antibody (GTX636247) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX636247) diluted at 1:2000.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/Singapore/63/04 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below.
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below.
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 μg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247

GTX636247 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX636247).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody (GTX636247) diluted at 1:100.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL).

Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636247. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza A virus NP (nucleoprotein) antibody (GTX135903-pro) (50 ng). Coated protein was probed with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

EC50 : 37.93 pM

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Influenza A viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987)) using antibodies as below.
Capture : Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (1 μg/mL).

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247

GTX636247 Lateral Flow Image

Detection of influenza A virus nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody (GTX636247 [HL1089])
Detection: Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])

Samples (1 ng) :
1. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
2. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
3. Lysis buffer

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247

GTX636247 Lateral Flow Image

Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Influenza A viral lysate (0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636247

GTX636247 IHC-P (cell pellet) Image

Influenza A virus Nucleoprotein antibody [HL1089] detects Influenza A virus Nucleoprotein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded flu A_NP (Kansas/2017/H3N2) transfected 293T cell FFPE Cell Pellet Block.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody [HL1089] (GTX636318) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Wang X et al. Virulence 2024; 15 (1) : 2301242 Immunosuppressants exert antiviral effects against influenza A(H1N1)pdm09 virus via inhibition of nucleic acid synthesis, mRNA splicing, and protein stability.
H Yang et al. J Hepatocell Carcinoma 2024; An Engineered Influenza a Virus Expressing the Co-Stimulator OX40L as an Oncolytic Agent Against Hepatocellular Carcinoma
实验应用 : WB, ICC/IF
Zhao X et al. Viruses 2021; 13 (8) 3-Indoleacetonitrile Is Highly Effective in Treating Influenza A Virus Infection In Vitro and In Vivo.
实验应用 : WB
我要回报文献

产品评论

Influenza A virus Nucleoprotein antibody [HL1089] 目录号 GTX636247

Rating
( Average 5 based on 2 users reviews)
实验应用
Lateral Flow(Lateral Flow)
( Average 5 based on 2 users reviews)
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Date : Anonymous submitted on 28-Sep-2021
Score :
Application Tested : Lateral Flow
Figure Note : Detection of recombinant influenza A virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
Detection: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Date : Anonymous submitted on 03-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : Influenza A virus
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)
Figure Note : Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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