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Influenza A virus Nucleoprotein抗体

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in IHC (Paraffin sections) (IHC-P). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot and Immunoprecipitation (WB IP). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

目录号 GTX125989

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA

种属反应

Influenza A virus
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX629633, Detection: GTX125989 or Capture: GTX125989, Detection: GTX629633

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

DF1 (Influenza A virus treatment for 10hr)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Influenza A virus

免疫原

Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11168364

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Influenza A Virus Nucleoprotein , Influenza A Virus NP

研究领域

產品图片

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) H1299 whole cell extract (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) DF1 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Influenza A viral lysate (1 ug & 1ng μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Influenza A (H1N1) viral lysate (0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

Influenza A virus Nucleoprotein antibody detects Influenza A virus Nucleoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Influenza A virus lysate (H1N1) infected 293T whole cell extract was separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

Immunofluorescent analysis of Influenza virus infected cells using Influenza A virus Nucleoprotein antibody antibody (GTX125989).
Sample: Influenza A and B virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody antibody (GTX125989) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Oncotarget in 2018. PMID: 29492200

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Front Immunol in 2017. PMID: 29312300

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Front Immunol in 2017. PMID: 29312300

Anti-Influenza A virus Nucleoprotein antibody used in IHC (Paraffin sections) (IHC-P). GTX125989

GTX125989 IHC-P Image

The data was published in the journal Virol J in 2020. PMID: 32321537

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Virol J in 2020. PMID: 32321537

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Front Immunol in 2018.PMID: 29904383

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the 2022 in Cell Death Discov. PMID: 35246529

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28842649

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Sci Rep in 2015.PMID: 26030097

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Front Immunol in 2018.PMID: 29904383

Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

The data was published in the journal Front Immunol in 2017. PMID: 29312300

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot and Immunoprecipitation (WB IP). GTX125989

GTX125989 WB IP Image

The data was published in the journal Front Immunol in 2017. PMID: 29312300

Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

The data was published in the journal Front Immunol in 2017. PMID: 29312300

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Front Immunol in 2017. PMID: 29312300

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

The data was published in the journal Front Immunol in 2018.PMID: 29904383

文献引用

产品评论

Influenza A virus Nucleoprotein antibody 目录号 GTX125989

Rating ( Average 5 based on 3 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
条带说明 :
Left : A549 cells were transfected with pcDNA3-NP (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 14-May-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 5 μL
Blocking : 3% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:30000, 25°C, 4Hr
条带说明 : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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