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Influenza A virus PA protein抗体

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

目录号 GTX118991

文献引用 43
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Influenza A virus
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Influenza A virus PA protein 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:100-1:1000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

83 kDa. ( Note )

阳性对照

DF-1(WSN infected cell s)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Influenza A virus

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus PA (A/WSN/1933(H1N1)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10619959

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Influenza A Virus Polymerase acidic protein , Influenza A Virus PA

研究领域

產品图片

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (- μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (GTX118991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991

GTX118991 ICC/IF Image

Influenza A Virus PA antibody detects polymerase PA (influenza A H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: polymerase PA (influenza A H1N1) protein stained by Influenza A Virus PA antibody (GTX118991) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

Sample (60 μg of whole cell lysate)
A: DF-1(WSN infected cell lysates)
B: DF-1 cell lysates
GTX118991 diluted at 1:250
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

The data was published in the journal J Virol in 2018.PMID: 29118119

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

The data was published in the journal J Virol in 2018.PMID: 29118119

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

The data was published in the journal Sci Rep in 2015. PMID: 25652161

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

The data was published in the journal Sci Rep in 2015. PMID: 25652161

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991

GTX118991 ICC/IF Image

The data was published in the journal Sci Rep in 2015. PMID: 25652161

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28842649

文献引用

Application Reference

WK Cho et al. J Funct ?Foods 2024; 112 : 105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
实验应用 : ICC/IF
Sheppard CM et al. Nat Commun 2023; 14 (1) : 6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.
Han YJ et al. J Biomed Sci 2023; 30 (1) : 10 Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34) : e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
Sonja Fernbach et al. Cell Rep 2022; 38 (12) : 110549 Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.
实验应用 : WB
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产品评论

Influenza A virus PA protein antibody 目录号 GTX118991

Rating
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实验应用
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Chen-I Hsu submitted on 10-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
条带说明 :
Left : A549 cells were transfected with pcDNA3-PA (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 15-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:5000, 25°C, 4Hr
条带说明 : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
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Application Reference
WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
实验应用 : ICC/IF
Sheppard CM et al. Nat Commun 2023; 14 (1):6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.
Han YJ et al. J Biomed Sci 2023; 30 (1):10 Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
Sonja Fernbach et al. Cell Rep 2022; 38 (12):110549 Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.
实验应用 : WB
Ma C et al. PLoS Pathog 2022; 18 (2):e1010295 p21 restricts influenza A virus by perturbing the viral polymerase complex and upregulating type I interferon signaling.
实验应用 : WB
Hongmin Sun et al. Engineering (Beijing) 2021; Multi-Omics-Guided Discovery of Omicsynins Produced by Streptomyces sp. 1647: Pseudo-Tetrapeptides Active Against Influenza A Viruses and Coronavirus HCoV-229E.
实验应用 : WB
Bdeir N et al. Sci Rep 2021; 11 (1):20477 Evidence that two instead of one defective interfering RNA in influenza A virus-derived defective interfering particles (DIPs) does not enhance antiviral activity.
实验应用 : WB
Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
实验应用 : WB
Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.
实验应用 : WB
Kwon EB et al. Molecules 2021; 26 (4) 8-O-(E-p-methoxycinnamoyl)harpagide Inhibits Influenza A Virus Infection by Suppressing Intracellular Calcium.
实验应用 : WB
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Sun N et al. Cell Rep 2020; 33 (5):108342 Type-IInterferon-Inducible SERTAD3 Inhibits Influenza A Virus Replication by Blocking the Assembly of Viral RNA Polymerase Complex.
实验应用 : WB
Esparza M et al. PLoS Pathog 2020; 16 (4):e1008407 Chemical intervention of influenza virus mRNA nuclear export.
Gao Q et al. J Virol 2020; 95 (2) Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.
实验应用 : WB
Arai Y et al. J Virol 2020; 95 (1) PA Mutations Inherited during Viral Evolution Act Cooperatively To Increase Replication of Contemporary H5N1 Influenza Virus with an Expanded Host Range.
实验应用 : WB
Hu J et al. Vet Microbiol 2020; 250:108838 PA-X protein of H5N1 avian influenza virus inhibits NF-kappaB activity, a potential mechanism for PA-X counteracting the host innate immune responses.
实验应用 : WB, ICC/IF
Zhang X et al. Front Microbiol 2020; 11:538355 Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.
实验应用 : WB
Hu A et al. Front Microbiol 2020; 11:178 Anthralin Suppresses the Proliferation of Influenza Virus by Inhibiting the Cap-Binding and Endonuclease Activity of Viral RNA Polymerase.
实验应用 : WB
Domingues P et al. Nat Commun 2019; 10 (1):3396 Profiling host ANP32A splicing landscapes to predict influenza A virus polymerase adaptation.
实验应用 : WB
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.
实验应用 : WB
Rodriguez-Frandsen A et al. J Virol 2019; (Epub) Viral determinants in H5N1 influenza A virus enable productive infection of HeLa cells.
实验应用 : WB
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
实验应用 : WB
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
实验应用 : WB
Arai Y et al. PLoS Pathog 2019; 15 (7):e1007919 PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.
实验应用 : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
实验应用 : WB
Zhao L et al. Mol Ther Nucleic Acids 2019; 14:509-519 The Downregulation of MicroRNA hsa-miR-340-5p in IAV-Infected A549 Cells Suppresses Viral Replication by Targeting RIG-I and OAS2.
实验应用 : WB
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
实验应用 : WB
Yang C et al. J Virol 2018; (Epub) The nucleolar protein LYAR facilitates ribonucleoprotein assembly of influenza A virus.
实验应用 : WB, ICC/IF
Mizuta S et al. ChemMedChem 2018; (Epub) Synthesis of Trifluoromethyl-α,β-unsaturated Lactones and Pyrazolinones and Discovery of Influenza Virus Polymerase Inhibitors.
实验应用 : WB
Wang J et al. Viruses 2018; 10 (6) Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus.
实验应用 : WB
Chen SC et al. J Virol 2017; 91 (20) Zinc Finger-Containing Cellular Transcription Corepressor ZBTB25 Promotes Influenza Virus RNA Transcription and Is a Target for Zinc Ejector Drugs.
Oymans J et al. J Virol 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
实验应用 : WB
Oymans J et al. bioRxiv 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
实验应用 : WB
Kuss-Duerkop SK et al. PLoS Pathog 2017; 13 (9):e1006635 Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.
Watanabe K et al. Sci Rep 2017; 7 (1):9500 Structure-based drug discovery for combating influenza virus by targeting the PA-PB1 interaction.
实验应用 : WB
Xu G et al. J Virol 2016; (Epub) Prevailing PA mutation K356R in avian influenza H9N2 virus increases mammalian replication and pathogenicity.
Gao H et al. Sci Rep 2015; 5:8262 The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses.
实验应用 : WB, ICC/IF
Su WC et al. J Virol 2015; 89 (22):11245-55 A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription.
实验应用 : WB
Hsu SF et al. J Virol 2015; 89 (7):3671-82 A Host Susceptibility Gene, DR1, Facilitates Influenza A Virus Replication by Suppressing Host Innate Immunity and Enhancing Viral RNA Replication.
实验应用 : WB
Roberts KL et al. J Virol 2014; Influenza A virus uses intercellular connections to spread to neighboring cells.
实验应用 : ICC/IF
Hsu WB et al. J Virol 2013; 87 (1):110-23 Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.
实验应用 : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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