Influenza A virus PB1 protein抗体

Name: Influenza A virus PB1 protein抗体
Brand: GeneTex
SKU: GTX125923
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

目录号 GTX125923

文献引用 56

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF
种属反应	Influenza A virus
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Influenza A virus PB1 protein 的产品

View product citations for antibody GTX125923 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

87 kDa. ( Note )

阳性对照

DF1 (WSN virus treatment for 10hr)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Influenza A virus

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2753122

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

Influenza A Virus Polymerase basic protein 1 , Influenza A Virus PB1

研究领域

產品图片

GTX125923 ICC/IF Image

Influenza A Virus PB1 antibody detects PB1 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB1 protein stained by Influenza A Virus PB1 antibody (GTX125923) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

GTX125923 WB Image

Influenza A Virus PB1 antibody detects protein by western blot analysis.
A. 5 μg DF1 whole cell lysate/extract (untreated)
B. 5 μg DF1 whole cell lysate/extract (WSN virus treatment for 10hr)
7.5% SDS-PAGE
Influenza A Virus PB1 antibody (GTX125923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125923 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (GTX125923) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125923 WB Image

The data was published in the journal J Virol in 2019. PMID: 31068418

GTX125923 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28842649

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 29118119

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 29118119

文献引用

Application Reference

WK Cho et al. J Funct ?Foods 2024; 112 : 105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection

实验应用 : ICC/IF

L Ma et al. Virol Sin 2023; S1995-820X (23) : 00136-0 Mutual antagonism of mouse-adaptation mutations in HA and PA proteins on H9N2 virus replication

Lamb CH et al. bioRxiv 2023; Cas13-based amplification-free quantification of aberrant RNAs during influenza virus infection.

Sheppard CM et al. Nat Commun 2023; 14 (1) : 6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.

Rigby CV et al. Virus Evol 2023; 9 (2) : vead056 Evolution of?transient RNA structure-RNA polymerase interactions in?respiratory RNA virus genomes.

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产品评论

Influenza A virus PB1 protein antibody 目录号 GTX125923

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 2 users reviews)

Date : Anonymous submitted on 19-Apr-2022

Score :

Application Tested : WB

Sample Species : Influenza A virus

Sample : MDCK cells with/without Influenza A virus (A/Puerto Rico/8/1934) infection

Amount used : 15μg

Blocking : 5% skim milk in 0.1% TBS-T, 20°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

Date : Chen-I Hsu submitted on 16-Sep-2021

Score :

Application Tested : WB

Sample Species : Influenza A virus

Sample : A549 cells

Amount used : 30μg

Blocking : 5% milk in TBST, 25°C, 1Hr

Primary Antibody : 1:2000, 4°C, 16Hr

Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr

条带说明 : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.

我要评论产品

Application Reference

WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection

实验应用 : ICC/IF

L Ma et al. Virol Sin 2023; S1995-820X (23):00136-0 Mutual antagonism of mouse-adaptation mutations in HA and PA proteins on H9N2 virus replication

Lamb CH et al. bioRxiv 2023; Cas13-based amplification-free quantification of aberrant RNAs during influenza virus infection.

Sheppard CM et al. Nat Commun 2023; 14 (1):6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.

Rigby CV et al. Virus Evol 2023; 9 (2):vead056 Evolution of?transient RNA structure-RNA polymerase interactions in?respiratory RNA virus genomes.

Yang H et al. PLoS Pathog 2023; 19 (7):e1011489 The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice.

Rigby C et al. bioRxiv 2023; Evolution of transient RNA structure-RNA polymerase interactions in respiratory RNA virus genomes.

Chen J et al. Virol Sin 2023; 38 (1):128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.

实验应用 : WB

G?nl F et al. Nat Commun 2023; 14 (1):787 The ubiquitination landscape of the influenza A virus polymerase.

实验应用 : WB, ICC/IF

Zheng H et al. J Virol 2022; 96 (12):e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.

实验应用 : WB

Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.

French H et al. Sci Adv 2022; 8 (36):eabp8655 Transient RNA structures cause aberrant influenza virus replication and innate immune activation.

Jianyuan Zhao et al. Nat Commun 2022; 13 (1):2079 An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.

实验应用 : WB

Sonja Fernbach et al. Cell Rep 2022; 38 (12):110549 Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.

实验应用 : WB

Ma C et al. PLoS Pathog 2022; 18 (2):e1010295 p21 restricts influenza A virus by perturbing the viral polymerase complex and upregulating type I interferon signaling.

实验应用 : WB

Junping Li et al. PLoS Pathog 2021; 17 (2):e1009336 Viral RNA-binding ability conferred by SUMOylation at PB1 K612 of influenza A virus is essential for viral pathogenesis and transmission.

实验应用 : WB, ICC/IF

Amol Patil et al. J Virol 2021; 95 (6) Phosphorylation of Influenza A Virus NS1 at Serine 205 Mediates Its Viral Polymerase-Enhancing Function.

实验应用 : WB

Vanessa Gerlt et al. Int J Mol Sci 2021; 22 (20) Cellular Protein Phosphatase 2A Regulates Cell Survival Mechanisms in Influenza A Virus Infection.

实验应用 : WB

Froggatt HM et al. PLoS Pathog 2021; 17 (9):e1009951 Influenza A virus segments five and six can harbor artificial introns allowing expanded coding capacity.

实验应用 : WB

Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.

实验应用 : WB

Deinhardt-Emmer S et al. Biomolecules 2021; 11 (6) Inhibition of Phosphatidylinositol 3-Kinase by Pictilisib Blocks Influenza Virus Propagation in Cells and in Lungs of Infected Mice.

实验应用 : WB

Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.

实验应用 : WB

Sarto J.D. et al. bioRxiv 2021; (Epub) Phosphorylation of JIP4 at S730 presents anti-viral properties against influenza A virus infection.

Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.

Sun N et al. Cell Rep 2020; 33 (5):108342 Type-IInterferon-Inducible SERTAD3 Inhibits Influenza A Virus Replication by Blocking the Assembly of Viral RNA Polymerase Complex.

实验应用 : WB

Gao Q et al. J Virol 2020; 95 (2) Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.

实验应用 : WB

Carrique L et al. Nature 2020; 587 (7835):638-643 Host ANP32A mediates the assembly of the influenza virus replicase.

实验应用 : WB

Chida J et al. PLoS Pathog 2020; 16 (8):e1008823 Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.

实验应用 : WB

Zhang X et al. Front Microbiol 2020; 11:538355 Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.

实验应用 : WB

Walker AP et al. J Virol 2020; 94 (13) Mutation of an Influenza Virus Polymerase 3' RNA Promoter Binding Site Inhibits Transcription Elongation.

实验应用 : WB

Hu A et al. Front Microbiol 2020; 11:178 Anthralin Suppresses the Proliferation of Influenza Virus by Inhibiting the Cap-Binding and Endonuclease Activity of Viral RNA Polymerase.

实验应用 : WB

Zhang T et al. Cell 2020; 180 (6):1115-1129 Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis.

实验应用 : WB

Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.

Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.

实验应用 : WB

Russell AB et al. J Virol 2019; 93 (14) Single-Cell Virus Sequencing of Influenza Infections That Trigger Innate Immunity.

Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.

实验应用 : WB

Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.

实验应用 : WB

Arai Y et al. PLoS Pathog 2019; 15 (7):e1007919 PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.

实验应用 : WB

Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.

实验应用 : WB

Zheng W et al. Cell Rep 2019; 27 (6):1875-1885.e5 Naproxen Exhibits Broad Anti-influenza Virus Activity in Mice by Impeding Viral Nucleoprotein Nuclear Export.

实验应用 : ICC/IF

Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.

实验应用 : WB

Mohl G et al. Antiviral Res 2019; 164:81-90 Novel influenza inhibitors designed to target PB1 interactions with host importin RanBP5.

Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.

实验应用 : WB

Nilsson-Payant BE et al. J Virol 2018; 92 (16) The Surface-Exposed PA 51-72-Loop of the Influenza A Virus Polymerase Is Required for Viral Genome Replication.

Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.

实验应用 : WB

Yang C et al. J Virol 2018; (Epub) The nucleolar protein LYAR facilitates ribonucleoprotein assembly of influenza A virus.

实验应用 : WB, ICC/IF

Wang J et al. Viruses 2018; 10 (6) Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus.

实验应用 : WB

Chen SC et al. J Virol 2017; 91 (20) Zinc Finger-Containing Cellular Transcription Corepressor ZBTB25 Promotes Influenza Virus RNA Transcription and Is a Target for Zinc Ejector Drugs.

Oymans J et al. J Virol 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.

实验应用 : WB

Oymans J et al. bioRxiv 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.

实验应用 : WB

Watanabe K et al. Sci Rep 2017; 7 (1):9500 Structure-based drug discovery for combating influenza virus by targeting the PA-PB1 interaction.

实验应用 : WB

Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.

实验应用 : WB

Du Y et al. MBio 2016; 7 (6) Annotating Protein Functional Residues by Coupling High-Throughput Fitness Profile and Homologous-Structure Analysis.

实验应用 : ICC/IF

Arai Y et al. PLoS Pathog 2016; 12 (4):e1005583 Novel Polymerase Gene Mutations for Human Adaptation in Clinical Isolates of Avian H5N1 Influenza Viruses.

实验应用 : WB

Su WC et al. J Virol 2015; 89 (22):11245-55 A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription.

实验应用 : WB

Hsu SF et al. J Virol 2015; 89 (7):3671-82 A Host Susceptibility Gene, DR1, Facilitates Influenza A Virus Replication by Suppressing Host Innate Immunity and Enhancing Viral RNA Replication.

实验应用 : WB

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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Influenza A virus PB1 protein抗体

目录号 GTX125923

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX125923 ICC/IF Image

GTX125923 WB Image

GTX125923 WB Image

GTX125923 WB Image

GTX125923 WB Image

GTX125923 WB Image

GTX125923 WB Image

GTX125923 WB Image

GTX125923 WB Image

文献引用

Application Reference

产品评论

Influenza A virus PB1 protein antibody 目录号 GTX125923

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