产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 0.05% BSA, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.61 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthesized peptide derived from human Insulin
纯化方式
Purified by antigen-affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10
细胞定位
Secreted
功能与背景
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
数据库
研究领域
產品图片
![Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826](/templates/default/js/lightbox/images/loading.gif "Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826")
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GTX02826 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [GT1229] (GTX02826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX02826 IHC-P Image
Insulin antibody [GT1229] detects Insulin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat pancreas.Insulin stained by Insulin antibody [GT1229] (GTX02826) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX02826 IHC-P Image
Insulin antibody [GT1229] detects Insulin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.Insulin stained by Insulin antibody [GT1229] (GTX02826) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX02826 WB Image
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [GT1229] (GTX02826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX02826 WB Image
WB analysis of various samples using GTX02826 Insulin antibody [GT1229].
Dilution : 1:1000
Loading : 25μg
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GTX02826 IHC-P Image
IHC-P analysis of rat pancreas tissue section using Insulin antibody [GT1229] Insulin antibody [GT1229].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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GTX02826 IHC-P Image
IHC-P analysis of mouse pancreas tissue section using Insulin antibody [GT1229] Insulin antibody [GT1229].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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GTX02826 IHC-P Image
IHC-P analysis of rat pancreas tissue (pancreatic islet) using GTX02826 Insulin antibody [GT1229].
Dilution : 1:100
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GTX02826 IHC-P Image
IHC-P analysis of mouse pancreas tissue (pancreatic islet) using GTX02826 Insulin antibody [GT1229].
Dilution : 1:100
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文献引用
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产品评论
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![Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826](/upload/website/prouct_img/small/GTX02826/GTX02826_4000000209_20210122_WB_M_pancreas_w_23053123_391.webp "Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/small/GTX02826/GTX02826_20210129_IHC-P_R_w_23053123_275.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/small/GTX02826/GTX02826_20210129_IHC-P_M_w_23053123_849.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826](/upload/website/prouct_img/small/GTX02826/GTX02826_4000000209_20210122_WB_R_pancreas_w_23053123_778.webp "Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826")
![Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826](/upload/website/prouct_img/small/GTX02826/CutImage_A19066_WB_01_(1068937)_w_23053123_191.webp "Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/small/GTX02826/A19066_IF_01_(1068664)_w_23053123_316.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/small/GTX02826/A19066_IF_02_(1068663)_w_23053123_720.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/small/GTX02826/A19066_IHC_01_(1068592)_w_23053123_307.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/small/GTX02826/A19066_IHC_02_(1068593)_w_23053123_210.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826](/upload/website/prouct_img/normal/GTX02826/GTX02826_4000000209_20210122_WB_M_pancreas_w_23053123_391.webp "Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/normal/GTX02826/GTX02826_20210129_IHC-P_R_w_23053123_275.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/normal/GTX02826/GTX02826_20210129_IHC-P_M_w_23053123_849.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826](/upload/website/prouct_img/normal/GTX02826/GTX02826_4000000209_20210122_WB_R_pancreas_w_23053123_778.webp "Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826")
![Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826](/upload/website/prouct_img/normal/GTX02826/CutImage_A19066_WB_01_(1068937)_w_23053123_191.webp "Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/normal/GTX02826/A19066_IF_01_(1068664)_w_23053123_316.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/normal/GTX02826/A19066_IF_02_(1068663)_w_23053123_720.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/normal/GTX02826/A19066_IHC_01_(1068592)_w_23053123_307.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
![Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826](/upload/website/prouct_img/normal/GTX02826/A19066_IHC_02_(1068593)_w_23053123_210.webp "Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826")
