产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| 1:100-1:5000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human insulin. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10
细胞定位
Secreted
功能与背景
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
数据库
研究领域
產品图片
![Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833](/templates/default/js/lightbox/images/loading.gif "Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833")
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GTX636833 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membranes were blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:3000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636833 IHC-P Image
Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636833 IHC-P Image
Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat pancreas.Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636833 IHC-P Image
Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.Sample: Paraffin-embedded human pancreas.Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636833 WB Image
Recombinant human insulin were separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636833 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833](/upload/website/prouct_img/small/GTX636833/GTX636833_44676_20231013_WB_M_pancreas_competitor_watermark_23101921_770.webp "Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833")
![Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833](/upload/website/prouct_img/small/GTX636833/GTX636833_T-44613_20220325_IHC-P_M_w_23061202_505.webp "Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833")
![Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833](/upload/website/prouct_img/small/GTX636833/GTX636833_T-44613_20220325_IHC-P_R_w_23061202_614.webp "Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833")
![Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833](/upload/website/prouct_img/small/GTX636833/GTX636833_T-44613_20220325_IHC-P_w_23061202_201.webp "Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833")
![Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833](/upload/website/prouct_img/small/GTX636833/GTX636833_44676_20230224_WB_Ag_23030219_911.webp "Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833")
![Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833](/upload/website/prouct_img/small/GTX636833/GTX636833_44676_20230224_WB_M_tissue_23030219_339.webp "Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833")
![Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833](/upload/website/prouct_img/normal/GTX636833/GTX636833_44676_20231013_WB_M_pancreas_competitor_watermark_23101921_770.webp "Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833")
![Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833](/upload/website/prouct_img/normal/GTX636833/GTX636833_T-44613_20220325_IHC-P_M_w_23061202_505.webp "Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833")
![Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833](/upload/website/prouct_img/normal/GTX636833/GTX636833_T-44613_20220325_IHC-P_R_w_23061202_614.webp "Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833")
![Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833](/upload/website/prouct_img/normal/GTX636833/GTX636833_T-44613_20220325_IHC-P_w_23061202_201.webp "Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833")
![Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833](/upload/website/prouct_img/normal/GTX636833/GTX636833_44676_20230224_WB_Ag_23030219_911.webp "Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833")
![Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833](/upload/website/prouct_img/normal/GTX636833/GTX636833_44676_20230224_WB_M_tissue_23030219_339.webp "Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833")
