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Integrin alpha 6抗体 [N3C2], Internal

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

目录号 GTX100565

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

127 kDa. ( Note )

阳性对照

Human ESC , rat lung , mouse lung

产品说明

Knockdown/Knockout validation was supported by customer review data.

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human Integrin alpha 6. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950600

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

integrin subunit alpha 6 , CD49f , ITGA6B , VLA-6

细胞定位

Membrane; Single-pass type I membrane protein

功能与背景

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 6 antibody [N3C2], Internal (GTX100565) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 6 antibody [N3C2], Internal (GTX100565) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

Sample (20 μg of whole cell lysate)
A: human ESC
5% SDS PAGE
GTX100565 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

The data was published in the journal Elife in 2019.PMID: 30801249

文献引用

产品评论

Integrin alpha 6 antibody [N3C2], Internal 目录号 GTX100565

Rating ( Average 4.3 based on 3 users reviews)
实验应用
Western Blot(WB) ( Average 4.3 based on 3 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 80 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:1500, 4°C, 16Hr
条带说明 : Lane 1: OC3 with siCtrl, Lane 2: OC3 with siXXXX, Lane 3: OC3-IV2 with siCtrl, Lane 4: OC3-IV2 with siXXXX
Date : Anonymous submitted on 19-Aug-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 20μg
Blocking : 5% BSA in TBST, 25°C, 2Hr
Primary Antibody : 1:500, 4°C, 16Hr
条带说明 : Lane 1: MDA-MB-231 cells, Lane 2: knockdown of human Integrin alpha 6 in MDA-MB-231 cells
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 5% milk, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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