产品说明
摘要
Integrin beta-1 / CD29 antibody detects integrin beta-1 protein (ITGB1), also known as CD29, which forms heterodimeric receptors with at least ten integrin alpha proteins to mediate various aspects of cell adhesion and migration. These functions are essential for embryogenesis, tissue repair, immune function, and other crucial processes in multicellular organisms. Integrin beta-1 has a predicted molecular weight of 88 kDa and exists in multiple isoforms.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:20000 |
| Assay dependent |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human Integrin beta 1 / CD29 Intracellular domain.
预测种属反应
Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide encompassing a sequence within the Intracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910031
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB
细胞定位
Cell membrane,Cell junction
功能与背景
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/templates/default/js/lightbox/images/loading.gif "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636657 WB Image
Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.Sample: Paraffin-embedded dog tissues.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX636657 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (100 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_44606_20220812_WB_competitor_watermark_22081423_881.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_44606_20221209_WB_D_C_22121123_828.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_44606_20220311_WB_w_23061202_489.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_T-44522_20220114_WB_KO_watermark_w_23061202_282.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_T-44522_20220114_IHC-P_M_1_w_23061202_148.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_T-44522_20220114_IHC-P_M_w_23061202_172.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_44606_20230217_IHC-P_Dog_23030219_310.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_44606_20220812_WB_TPM_watermark_22081423_694.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_44606_20220819_WB_B_22082402_449.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/small/GTX636657/GTX636657_44606_20221227_IHC-P_multiple_M_22122821_698.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_44606_20220812_WB_competitor_watermark_22081423_881.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_44606_20221209_WB_D_C_22121123_828.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_44606_20220311_WB_w_23061202_489.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_T-44522_20220114_WB_KO_watermark_w_23061202_282.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_T-44522_20220114_IHC-P_M_1_w_23061202_148.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_T-44522_20220114_IHC-P_M_w_23061202_172.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_44606_20230217_IHC-P_Dog_23030219_310.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_44606_20220812_WB_TPM_watermark_22081423_694.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_44606_20220819_WB_B_22082402_449.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657")
![Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657](/upload/website/prouct_img/normal/GTX636657/GTX636657_44606_20221227_IHC-P_multiple_M_22122821_698.webp "Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657")
