产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:20000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human Integrin beta 1 / CD29 Intracellular domain.
预测种属反应
Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide encompassing a sequence within the Intracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910032
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB
细胞定位
Cell membrane,Cell junction
功能与背景
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/templates/default/js/lightbox/images/loading.gif "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
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GTX636658 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636658 ICC/IF Image
Integrin beta 1 / CD29 antibody [HL1256] detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636658 WB Image
Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636658 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636658 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (100 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636658 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/small/GTX636658/GTX636658_44606_20220812_WB_competitor_watermark_22081423_710.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658](/upload/website/prouct_img/small/GTX636658/GTX636658_44606_20220916_ICC_IF_22102723_719.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/small/GTX636658/GTX636658_T-44522_20220114_WB_KO_watermark_w_23061202_413.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/small/GTX636658/GTX636658_44606_20220311_WB_w_23061202_183.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/small/GTX636658/GTX636658_44606_20220819_WB_B_22082402_904.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/small/GTX636658/GTX636658_44606_20221209_WB_D_C_22121123_173.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/normal/GTX636658/GTX636658_44606_20220812_WB_competitor_watermark_22081423_710.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658](/upload/website/prouct_img/normal/GTX636658/GTX636658_44606_20220916_ICC_IF_22102723_719.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/normal/GTX636658/GTX636658_T-44522_20220114_WB_KO_watermark_w_23061202_413.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/normal/GTX636658/GTX636658_44606_20220311_WB_w_23061202_183.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/normal/GTX636658/GTX636658_44606_20220819_WB_B_22082402_904.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
![Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658](/upload/website/prouct_img/normal/GTX636658/GTX636658_44606_20221209_WB_D_C_22121123_173.webp "Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658")
