产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
human ITGB1-transfected 293T , HeLa
预测种属反应
Rat, Sheep, Bovine, Cat, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10722978
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB
细胞定位
Cell membrane; Single-pass type I membrane protein , Melanosome
功能与背景
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX112971 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
GTX112971 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX112971 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX112971 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brown adipocyte.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX112971 WB Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human Integrin beta 1 / CD29-transfected 293T cells 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody (GTX112971) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX112971 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.
|
|
GTX112971 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
GTX112971 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
文献引用
产品评论
目前没有使用 Integrin beta 1 / CD29 antibody (GTX112971) 的产品评论被收录。快发表第一篇产品评论吧!