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Involucrin抗体 [N1N2], N-term

Overexpression
Anti-Involucrin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116012
Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012
Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012
Anti-Involucrin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116012

目录号 GTX116012

文献引用 7

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Involucrin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

阳性对照

human IVL-transfected 293T cells

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.72 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Involucrin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10626126

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

involucrin

功能与背景

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Involucrin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116012

GTX116012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Involucrin(GTX116012) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012

GTX116012 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012

GTX116012 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Involucrin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116012

GTX116012 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Involucrin(GTX116012) antibody at 1:200 dilution.

文献引用

Application Reference

Usuki S et al. Biology 2022; 11 (1) : 121 Konjac Ceramide (kCer)-Mediated Signal Transduction of the Sema3A Pathway Promotes HaCaT Keratinocyte Differentiation.
Colombo I et al. Mediators Inflamm 2017; 2017 : 7435621 HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the Inflammatory/Repair Response of Human Keratinocytes.
实验应用 : WB
种属反应 : Human
Colombo I et al. Hindawi 2017; : Epub ahead of print HaCaT cells as a reliable in vitro differentiation model to dissect the inflammatory/repair response of human keratinocytes.
实验应用 : WB
种属反应 : Human
Qian L et al. Cytokine 2017; 96 : 114-122 LncRNA expression profile of ΔNp63α in cervical squamous cancers and its suppressive effects on LIF expression.
Dang NN et al. Braz J Med Biol Res 2015; 48 (1) : 39-45 Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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