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Japanese encephalitis virus Core protein C抗体

Anti-Japanese encephalitis virus Core protein C antibody used in Western Blot (WB). GTX131368
Anti-Japanese encephalitis virus Core protein C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131368

目录号 GTX131368

文献引用 3
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Japanese encephalitis virus
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:3000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

12 kDa. ( Note )

阳性对照

JEV infected BHK-21 cells

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Japanese encephalitis virus

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Core protein C (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886468

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Japanese encephalitis Virus Core , JEV Core

功能与背景

The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. Non-structural protein 2B is a required cofactor for the serine protease function of NS3. Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions.

研究领域

產品图片

Anti-Japanese encephalitis virus Core protein C antibody used in Western Blot (WB). GTX131368

GTX131368 WB Image

Core protein C (JEV) antibody detects Core protein C (JEV) protein by western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Core protein C (JEV) antibody (GTX131368) at a dilution of 1:3000 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Japanese encephalitis virus Core protein C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131368

GTX131368 ICC/IF Image

Core protein C (Japanese encephalitis virus) antibody detects Core protein C (Japanese encephalitis virus) by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol.
Green: Core protein C (Japanese encephalitis virus) stained by core protein C (Japanese encephalitis virus) antibody (GTX131368) diluted at 1:3000.
Blue: Hoechst 33342 staining.

文献引用

Application Reference

Xiaochun Ma et al. Viruses 2022; 14 (4) Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection.
Sapna Sehrawat et al. J Virol 2021; Valosin-containing protein/p97 plays critical roles in the Japanese encephalitis virus life cycle.
实验应用 : WB, ICC/IF
Sarkar R et al. J Gen Virol 2020; (Epub) Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets.
实验应用 : WB, ICC/IF
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产品评论

Japanese encephalitis virus Core protein C antibody 目录号 GTX131368

Rating
( Average 3 based on 2 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 3 based on 2 users reviews)
Anti-Japanese encephalitis virus Core protein C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131368
Date : Anonymous submitted on 04-Oct-2016
Score :
Application Tested : ICC/IF
Sample Species : JEV
Sample : JEV infected BHK21 cells (MOI=0.1 48h)
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Japanese encephalitis virus Core protein C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131368
Date : Anonymous submitted on 04-Oct-2016
Score :
Application Tested : ICC/IF
Sample Species : JEV
Sample : JEV infected TE671 cells
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : A: TE671 cells, B: JEV infected TE671 cells. (MOI=0.1 48h)
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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