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Japanese encephalitis virus NS3抗体

Orthogonal
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868
Anti-Japanese encephalitis virus NS3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125868

目录号 GTX125868

文献引用 24

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Japanese encephalitis virus
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Japanese encephalitis virus NS3 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

69 kDa. ( Note )

阳性对照

Japanese encephalitis virus infected BHK-21 cells

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Japanese encephalitis virus

免疫原

Recombinant protein encompassing a sequence within the center region of NS3 (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11177224

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Japanese encephalitis Virus Non-structural protein 3 , Japanese encephalitis Virus NS3 , JEV Non-structural protein 3 , JEV NS3

研究领域

產品图片

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extract (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Japanese encephalitis virus NS3 antibody (GTX125868) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by western blot analysis.
A. 10 μg BHK-21 whole cell extract
B. 10 μg whole cell extract of Japanese encephalitis virus infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
10% SDS-PAGE
NS3 (Japanese encephalitis virus) antibody (GTX125868) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868

GTX125868 ICC/IF Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in paraformaldehyde.
Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody (GTX125868) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Japanese encephalitis virus NS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125868

GTX125868 ICC/IF Image

NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH.
Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody (GTX125868) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in Western Blot (WB). GTX125868

GTX125868 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28349961

Anti-Japanese encephalitis virus NS3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125868

GTX125868 IHC-Fr Image

The data was published in the journal Nat Commun in 2019. PMID: 30742008

文献引用

Application Reference

Z Chen et al. Virol J 2023; 20 (1) : 280 Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells
Moon JH et al. BMC Neurosci 2023; 24 (1) : 59 Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways.
Yang S et al. Front Cell Infect Microbiol 2023; 13 : 1239234 Japanese encephalitis virus perturbs PML-nuclear bodies by engaging in interactions with distinct porcine PML isoforms.
Hong JM et al. Virus Res 2023; 338 : 199249 Antiviral activity of prion protein against Japanese encephalitis virus infection in vitro and in vivo.
Liang Z et al. Viruses 2023; 15 (7) Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection.
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Application Reference
Z Chen et al. Virol J 2023; 20 (1):280 Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells
Moon JH et al. BMC Neurosci 2023; 24 (1):59 Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways.
Yang S et al. Front Cell Infect Microbiol 2023; 13:1239234 Japanese encephalitis virus perturbs PML-nuclear bodies by engaging in interactions with distinct porcine PML isoforms.
Hong JM et al. Virus Res 2023; 338:199249 Antiviral activity of prion protein against Japanese encephalitis virus infection in vitro and in vivo.
Liang Z et al. Viruses 2023; 15 (7) Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection.
Xie S et al. Microbiol Spectr 2022; 10 (4):e0114722 Japanese Encephalitis Virus (JEV) NS1' Enhances the Viral Infection of Dendritic Cells (DCs) and Macrophages in Pig Tonsils.
Chenxi Li et al. Int. J. Mol. Sci. 2022; 23 (24) A Recombinant Genotype I Japanese Encephalitis Virus Expressing a Gaussia Luciferase Gene for Antiviral Drug Screening Assay and Neutralizing Antibodies Detection
实验应用 : WB
Xiaolong Zhou et al. Virol J 2021; 18 (1):29 Inhibition of Japanese encephalitis virus proliferation by long non-coding RNA SUSAJ1 in PK-15 cells.
实验应用 : WB
Zhu J et al. Front Vet Sci 2021; 8:757978 Molecular Cloning of Alternative Splicing Variants of the Porcine PML Gene and Its Expression Patterns During Japanese Encephalitis Virus Infection.
实验应用 : WB
Bhaskar M et al. mBio 2021; 12 (6):e0271221 Involvement of RIG-I Pathway in Neurotropic Virus-Induced Acute Flaccid Paralysis and Subsequent Spinal Motor Neuron Death.
实验应用 : IHC-P
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
实验应用 : WB
Xie S et al. Virol Sin 2021; (Epub) Japanese Encephalitis Virus NS2B-3 Protein Complex Promotes Cell Apoptosis and Viral Particle Release by Down-Regulating the Expression of AXL.
Yu D et al. Virol Sin 2021; (Epub) C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein.
Zhao C et al. Nat Commun 2020; 11 (1):5178 CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication.
Kumar S et al. ACS Chem Neurosci 2020; 11 (21):3683-3696 Antiviral Activity of Belladonna During Japanese Encephalitis Virus Infection via Inhibition of Microglia Activation and Inflammation Leading to Neuronal Cell Survival.
实验应用 : WB
Hsieh JT et al. Nat Commun 2019; 10 (1):706 Japanese encephalitis virus neuropenetrance is driven by mast cell chymase.
Cui X et al. Viruses 2019; 11 (6) Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro.
实验应用 : WB
Carr M et al. Virus Genes 2019; (Epub) Upregulated expression of the antioxidant sestrin 2 identified by transcriptomic analysis of Japanese encephalitis virus-infected SH-SY5Y neuroblastoma cells.
实验应用 : WB
Suvadip Mallick et al. bioRxiv 2019; (Epub) Novel strategy for treating neurotropic viral infections using hypolipidemic drug Atorvastatin.
实验应用 : WB, ICC/IF
Lien JC et al. Sci Rep 2018; 8 (1):16595 Structure analysis and antiviral activity of CW-33 analogues against Japanese encephalitis virus.
实验应用 : ICC/IF
Chen M et al. Sci Rep 2017; 7 (1):15931 Broad-spectrum antiviral agents: secreted phospholipase A2 targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane.
Lu CY et al. Viruses 2017; 9 (4) Single-Round Infectious Particle Antiviral Screening Assays for the Japanese Encephalitis Virus.
实验应用 : ICC/IF
Xu Q et al. Sci Rep 2017; 7:45375 E3 Ubiquitin Ligase Nedd4 Promotes Japanese Encephalitis Virus Replication by Suppressing Autophagy in Human Neuroblastoma Cells.
实验应用 : WB
Fan W et al. Sci Rep 2016; 6:33698 TRIM52 inhibits Japanese Encephalitis Virus replication by degrading the viral NS2A.
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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