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Japanese encephalitis virus NS5抗体

Anti-Japanese encephalitis virus NS5 antibody used in Western Blot (WB). GTX131359
Anti-Japanese encephalitis virus NS5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131359

目录号 GTX131359

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF

种属反应

Japanese encephalitis virus
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:3000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

103 kDa. ( Note )

阳性对照

JEV infected BHK-21 cells

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Japanese encephalitis virus

免疫原

Recombinant protein encompassing a sequence within the center region of NS5 (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886466

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Japanese encephalitis Virus Non-structural protein 5A , Japanese encephalitis Virus NS5A , JEV Non-structural protein 5A , JEV NS5A

功能与背景

The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. Non-structural protein 2B is a required cofactor for the serine protease function of NS3. Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions.

研究领域

產品图片

Anti-Japanese encephalitis virus NS5 antibody used in Western Blot (WB). GTX131359

GTX131359 WB Image

NS5 (JEV) antibody detects NS5 (JEV) protein by Western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NS5 (JEV) antibody (GTX131359) at a dilution of 1:3000.

Anti-Japanese encephalitis virus NS5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131359

GTX131359 ICC/IF Image

NS5 protein (Japanese encephalitis virus) antibody detects NS5 protein (Japanese encephalitis virus) by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol.
Green: NS5 (Japanese encephalitis virus) protein stained by NS5 protein (Japanese encephalitis virus) antibody (GTX131359) diluted at 1:3000.
Blue: Hoechst 33342 staining.

文献引用

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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