产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000-1:10000 |
Assay dependent |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B
细胞定位
Nucleus
功能与背景
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
|
GTX637013 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (GTX637013) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX637013 IHC-P Image
KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX637013 IHC-P Image
KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX637013 IHC-P Image
KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX637013 WB Image
Various whole cell extract (30 μg) were separatedby 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (GTX637013) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX637013 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (GTX637013) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX637013 IHC-P Image
KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX637013 ICC/IF Image
KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
|
文献引用
目前没有使用 KAP1 antibody [HL1539] (GTX637013) 的文献被收录。快来回报我们第一篇参考文献吧!
产品评论
目前没有使用 KAP1 antibody [HL1539] (GTX637013) 的产品评论被收录。快发表第一篇产品评论吧!